Seeding density, I would suggest go in the high range suggested, either 96w or 384w plate format. I used FCCP and CCCP to test kit and both compounds work very nice, there are more efficacious if you do stimulation in PBS. On the reads I used Cytation 3 and I have to play so much to get right reads!...In my hands did not work auto gain, I have to play with it and set it at 150 gain.
In my third run I could see the clear effect in a dose-response manner in two cell lines tested. Worth using it.
However I tested JC-10 in paralel and in one run I got similar results with the not so easy NIR kit.
Anabel de la Garza
Submitted Dec 24 2019