Overview

  • Product name
  • Description
    Goat polyclonal to NIR2
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide:

    KARSISLKLDSEE

    , corresponding to C terminal amino acids 1232-1244 of Human NIR2.

Properties

Applications

Our Abpromise guarantee covers the use of ab22823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC: 1/200 - please refer to abreview for further details.
    WB: See Abreview published on 11th April, 2007 for further information.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Regulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Binds phosphatidyl inositol phosphates (in vitro). May catalyze the transfer of phosphatidylinositol and phosphatidylcholine between membranes (By similarity). Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the PtdIns transfer protein family. PI transfer class IIA subfamily.
      Contains 1 DDHD domain.
    • Post-translational
      modifications
      Phosphorylated on multiple sites by CDK1 at the onset of mitosis. Phosphorylation facilitates dissociation from the Golgi complex and is required for interaction with PLK1.
      Phosphorylated on threonine residues upon treatment with oleic acid.
      Phosphorylated on tyrosine residues by PTK2B.
    • Cellular localization
      Cytoplasm. Golgi apparatus > Golgi stack membrane. Endoplasmic reticulum membrane. Lipid droplet. Cleavage furrow. Midbody. Peripheral membrane protein associated with Golgi stacks in interphase cells. A minor proportion is associated with the endoplasmic reticulum. Associated with lipid droplets. Dissociates from the Golgi early on in mitosis and localizes to the cleavage furrow and midbody during cytokinesis.
    • Information by UniProt
    • Database links
    • Alternative names
      • DRES9 antibody
      • Drosophila retinal degeneration B antibody
      • Drosophila retinal degeneration B homolog antibody
      • FLJ44997 antibody
      • membrane-associated 1 antibody
      • Membrane-associated phosphatidylinositol transfer protein 1 antibody
      • NIR 2 antibody
      • NIR-2 antibody
      • NIR2 antibody
      • OTTHUMP00000236414 antibody
      • OTTHUMP00000236415 antibody
      • Phosphatidylinositol transfer protein antibody
      • phosphatidylinositol transfer protein membrane associated antibody
      • PITM1_HUMAN antibody
      • PITPnm 1 antibody
      • PITPNM antibody
      • Pitpnm1 antibody
      • PYK2 N terminal domain interacting receptor 2 antibody
      • Pyk2 N-terminal domain-interacting receptor 2 antibody
      • RD 9 antibody
      • RD9 antibody
      • RDGB antibody
      • RDGB1 antibody
      • RDGBA1 antibody
      • retinal degeneration B alpha 1 antibody
      see all

    Images

    • ab22823 (1/200) staining NIR2 in mouse macrophage cells by ICC/IF. Secondary antibody: donkey polyclonal to goat IgG conjugated to Alexa Fluor® 594; Counterstain: DAPI (blue) - please refer to abrevew for further details.

      See Abreview

    References

    This product has been referenced in:
    • Keinan O  et al. The lipid-transfer protein Nir2 enhances epithelial-mesenchymal transition and facilitates breast cancer metastasis. J Cell Sci 127:4740-9 (2014). Read more (PubMed: 25179602) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF10A)
    Loading amount
    50 µg
    Specification
    MCF10A
    Gel Running Conditions
    Reduced Denaturing (7.5% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Sep 05 2012

    Answer

    Thank you very much for your reply.


    I am pleased to hear you would like to make use of our testing discount offer to test ab22823NIR2 antibodyin human samples. I can provide the following discount code.

    DISCOUNT CODE: #####
    Expiration date: ######

    To use this code:

    1. Place your order with Zotal, including this discount code. This order will not be discounted, but you will need to provide the code.

    2. Once you receive the antibody, test ab22823in human samples.

    3. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    4. After the review is submitted to us, the discount code becomes active. Simply place your new order with Zotal and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    The code will give you: 1 freeprimary antibody after submission of the Abreivewbefore the expiration date. To redeem this offer, please submit an Abreviewforhuman samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed by placing your order with Zotal, and include the discount code.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative.

    I have copied this email to Zotal so they are aware you have been provided this code by us. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    Read More

    Answer

    Thank you for your reply

    I confirm can offer a discount off a future purchase if you buy ab22823 now, test it in human samples and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab22823 in human samples. I will then send a discount code, and copy this to Zotal so they are aware. This code must be issued before purchasing ab22823 so please wait for my reply before ordering.

    2. Purchase ab22823 from Zotal, and let them have the discount code when you place your order.

    3. Test it in human.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order with Zotal and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab22823 turns out to be unsuitable for human samples, you will still receive the discount on your next purchase after your Abreview has been submitted.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Please let me know if you have any questions about this offer and I would be happy to help you further. Once you confirm, I can send you the discount code.

    Read More

    Answer

    Thank you for contacting us and for your interest in our products.

    I am sorry to confirm that as far as we are aware, ab22823 has never been tested with samples from human. All tested species cross-reactivity information covered by our guarantee is stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

    The immunogen is from the human protein, which means it is very likely this antibody will detect in human samples, and therefore human is listed as a predicted species on the datasheet. However, I am sorry we are not able to guarantee this without further testing.

    If you would like to test the ab22823 inhuman samples, please contactme againby replying to this message prior to the purchase as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    Regarding price, normally this can be found on the datasheet. However, I am sorry we do not sell directly to Israel. I can recommend to contact Zotal, our distributor in Israel, for information regarding price.


    Zotal Ltd
    4 Habarzel St.
    Tel Aviv
    69710
    Israel Tel+ 972 3 6492444
    Websitehttp://www.zotal.co.il
    Fax+ 972 3 6496664
    mailto:Emailsales@zotal.co.il

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse macrophage cell line)
    Loading amount
    50 µg
    Specification
    mouse macrophage cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

    Abcam user community

    Verified customer

    Submitted Apr 11 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (MOUSE MACROPHAGE CELL LINES)
    Specification
    MOUSE MACROPHAGE CELL LINES
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1.5%

    Abcam user community

    Verified customer

    Submitted Mar 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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