Key features and details
- Rabbit polyclonal to NIR2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NIR2 antibody
See all NIR2 primary antibodies
DescriptionRabbit polyclonal to NIR2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human fallopian tube tissue. WB: RT4 cell lysate. ICC/IF: A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 135 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRegulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Binds phosphatidyl inositol phosphates (in vitro). May catalyze the transfer of phosphatidylinositol and phosphatidylcholine between membranes (By similarity). Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions.
Sequence similaritiesBelongs to the PtdIns transfer protein family. PI transfer class IIA subfamily.
Contains 1 DDHD domain.
modificationsPhosphorylated on multiple sites by CDK1 at the onset of mitosis. Phosphorylation facilitates dissociation from the Golgi complex and is required for interaction with PLK1.
Phosphorylated on threonine residues upon treatment with oleic acid.
Phosphorylated on tyrosine residues by PTK2B.
Cellular localizationCytoplasm. Golgi apparatus > Golgi stack membrane. Endoplasmic reticulum membrane. Lipid droplet. Cleavage furrow. Midbody. Peripheral membrane protein associated with Golgi stacks in interphase cells. A minor proportion is associated with the endoplasmic reticulum. Associated with lipid droplets. Dissociates from the Golgi early on in mitosis and localizes to the cleavage furrow and midbody during cytokinesis.
- Information by UniProt
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Paraffin-embedded human fallopian tube tissue stained for NIR2 using ab254959 at 1/200 dilution in immunohistochemical analysis.
Lane 2 : Anti-NIR2 antibody (ab254959) at 0.4 µg/ml
Lane 1 : DNA Ladder
Lane 2 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Predicted band size: 135 kDa
PFA fixed, Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling NIR2 using ab254959 at 4µg/ml (green) in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254959 has not yet been referenced specifically in any publications.