Product nameAnti-NIRF antibody
DescriptionRabbit polyclonal to NIRF
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
- Jurkat cell lysate (WB), human heart (IHC-P).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab28673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 90 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
FunctionE3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of PCNP. May participate in methylation-dependent transcriptional regulation. Important for G1/S transition. Overexpression causes G1 phase cell arrest.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 PHD-type zinc finger.
Contains 2 RING-type zinc fingers.
Contains 1 ubiquitin-like domain.
Contains 1 YDG domain.
modificationsMay be autoubiquitinated; which may lead to proteasomal degradation.
Phosphorylated. Phosphorylation may be mediated by CDK2.
- Information by UniProt
- DKFZp434B0920 antibody
- DKFZp686G0837 antibody
- E3 ubiquitin-protein ligase UHRF2 antibody
Anti-NIRF antibody (ab28673) at 0.5 µg/ml + Jurkat Cell Lysate with 5% skim milk / PBS buffer
HRP conjugated anti-Rabbit IgG1: 50,000 - 100,000 dilution.
Observed band size: 90 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
IHC staining of paraffin embedded human heart with ab28673 at 4.0-8.0 ug/ml. Positive staining (arrows) is myocardial cells. Magnification: x400
Western blot analysis using ab28673 at 1/2000.
Lane 1) Whole cell HeLa lysate
Lane 2) Recombinant purified UHRF2 protein
Lane 3) Recombinant purified UHRF1 protein
Lane 4) Whole cell HeLa lysate with UHRF1 knockdown (short hairpin RNA)
A HRP-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
This product has been referenced in:
- Wang Y et al. UHRF2 promotes DNA damage response by decreasing p21 via RING finger domain. Biotechnol Lett N/A:N/A (2018). Read more (PubMed: 29923055) »
- Zhang T et al. UHRF2 decreases H3K9ac expression by interacting with it through the PHD and SRA/YDG domain in HepG2 hepatocellular carcinoma cells. Int J Mol Med 39:126-134 (2017). Human . Read more (PubMed: 28004105) »