Key features and details
- Rabbit polyclonal to NISCH
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NISCH antibody
See all NISCH primary antibodies
DescriptionRabbit polyclonal to NISCH
Specificityab240588 has not performed satisfactorily when used for WB of NISCH in crude preparations (e.g. whole cell lysate).This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NISCH.
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human NISCH aa 1100-1150. The exact sequence is proprietary.
Database link: Q9Y2I1
- IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab240588 was affinity purified using an epitope specific to NISCH immobilized on solid support.
Our Abpromise guarantee covers the use of ab240588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceMouse Nischarin (NISCH) has been shown to interact with the alpha-5 subunit of integrin and inhibit cell migration. Its human homologue (imidazoline receptor antisera-selected (IRAS)), contains an NH2-terminal extension and is a larger protein of 1504 amino acids consisting of an NH2-terminal PX domain, 5 putative leucine-rich repeats, a predicted coiled-coil domain, and a long COOH-terminal region. It has the ability to homo-oligomerize via its coiled-coil region. The PX domain of IRAS is essential for association with phosphatidylinositol 3-phosphate-enriched endosomal membranes. NISCH may serve as a functional imidazoline I1-receptor.
Cellular localizationCell membrane. Cytoplasm. Early endosome. Recycling endosome.
- FLJ14425 antibody
- FLJ40413 antibody
- FLJ90519 antibody
NISCH was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240588 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240588 at 0.4 µg/ml.
Lane 1: ab240588 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240588 has not yet been referenced specifically in any publications.