Key features and details
- Rabbit polyclonal to NIT1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NIT1 antibody
See all NIT1 primary antibodies
DescriptionRabbit polyclonal to NIT1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human NIT1 aa 1-243.
Database link: Q86X76
Storage bufferpH: 7.40
Constituents: Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237043 in the following tested applications.
|WB||1/500 - 1/5000. Predicted molecular weight: 35 kDa.|
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.
Tissue specificityDetected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
Contains 1 CN hydrolase domain.
Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
- MGC57670 antibody
- NIT1 antibody
- NIT1_HUMAN antibody
All lanes : Anti-NIT1 antibody (ab237043) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
Paraffin-embedded human cervical cancer tissue stained for NIT1 using ab237043 at 1/1200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for NIT1 (green) using ab237043 at 1/400 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237043 has not yet been referenced specifically in any publications.