Product nameNitric Oxide Assay Kit
See all Nitric oxide kits
Sample typeCell culture supernatant, Saliva, Urine, Serum, Plasma, Whole Blood, Tissue Extracts, Cell Lysate
Range0.6 µM - 200 µM
Nitric Oxide Assay Kit (ab272517) is a simple, direct and automation-ready procedure for measuring NO. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2 - /NO3 - as a measure for NO level. This Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pre-treatment and assay is reduced to as little as 30 minutes.
Sensitive and accurate: Detection range 0.6 - 200 µM in 96-well plate.
Rapid and reliable: Using an optimized VCl3 reagent, the time required for reduction of NO3 – to NO2 - is 10 min at 60°C.
Simple and high-throughput: The procedure involves mixing sample with three reagents, incubation for 10 minutes at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests NaOH 1 x 1ml Nitrite Standard (1 mM) 1 x 1ml Reagent A 1 x 12ml Reagent B 1 x 500µl Reagent C 1 x 12ml ZnSO4 1 x 1ml
RelevanceNitric oxide (NO) is a key vertebrate biological messenger, playing an important role in neurotransmission, vascular regulation, immune responses and apoptosis. NO , also known as "endothelium-derived relaxing factor" or "EDRF", is synthesized from L-arginine, oxygen and NADPH by various NO synthases. Most of the NO in the cell is oxidized to nitrite (NO2-) and nitrate (NO3-), and therefore the concentrations of these anions are generally as a quantitative measure of NO production.
Our Abpromise guarantee covers the use of ab272517 in the following tested applications.
|Functional Studies||Use at an assay dependent concentration.|
ab272517 has not yet been referenced specifically in any publications.