• Product name

    Anti-Nitro tyrosine antibody
    See all Nitro tyrosine primary antibodies
  • Description

    Rabbit polyclonal to Nitro tyrosine
  • Host species

  • Specificity

    This antibody reacts with free and bound nitrotyrosine. There is no cross reaction with free or bound tyrosine
  • Tested applications

    Suitable for: IHC-FoFr, IHC-P, WB, ELISAmore details
  • Species reactivity

    not applicable
  • Immunogen

    Chemical/ Small Molecule conjugated to KLH: Nitrotyrosine



Our Abpromise guarantee covers the use of ab42789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 22540017
IHC-P Use at an assay dependent concentration. PubMed: 24551153
WB 1/200 - 1/2000. Predicted molecular weight: 1 kDa.
ELISA 1/5000 - 1/20000.


  • Relevance

    Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
  • Alternative names

    • NO tyrosine antibody
    • nTyr antibody


  • All lanes: Whole cell lysate prepared from human penile tissue loaded at 30µg. Gel is 4-15% mini gel, performed under reduced, denaturing conditions.
    ab42789 added at a 1/1000 dilution.
    Secondary used was a donkey anti-rabbit IgG conjugated to HRP, used at a 1/7000 dilution.
  • Immunohistochemical analysis of prediabetic obese rat penile tissue, staining Nitro tyrosine with ab72789.

    Samples were blocked with 10% normal goat serum, permeabilized with 0.3% Triton X-100 before being incubated with primary antibody (1/100) for 48 hours at 4°C. A biotinylated goat anti-rabbit IgG (1/400) was used as the secondary antibody and staining was detected using DAB.


This product has been referenced in:

  • Trias E  et al. Emergence of Microglia Bearing Senescence Markers During Paralysis Progression in a Rat Model of Inherited ALS. Front Aging Neurosci 11:42 (2019). Read more (PubMed: 30873018) »
  • Lee JA  et al. Age Induced Nitroso-Redox Imbalance Leads to Subclinical Hypogonadism in Male Mice. Front Endocrinol (Lausanne) 10:190 (2019). Read more (PubMed: 30984111) »
See all 11 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for calling Abcam earlier today.

As we discussed, I am sending you a new vial of antibody, ab42789 as a free of charge replacement. Your new order number is *********.

The antibody is covered under our Abpromise to work in western blot and the Abpromise is valid for 6 months after delivery. If you have any issues with this antibody, please contact us within that time frame to let us know.

I hope that your experiments go well.

Read More
Western blot
Human Tissue lysate - whole (Human Penile Tissue)
Loading amount
30 µg
Human Penile Tissue
Gel Running Conditions
Reduced Denaturing (4-15% mini gel)
Blocking step
Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Miss. Gwen Lagoda

Verified customer

Submitted Apr 25 2011

Western blot
Mouse Tissue lysate - whole (Penile Tissue)
Loading amount
20 µg
Penile Tissue
Gel Running Conditions
Reduced Denaturing (4-15 % mini gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Miss. Gwen Lagoda

Verified customer

Submitted Feb 26 2009

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