Key features and details
- Goat polyclonal to Nitro tyrosine (Biotin)
- Suitable for: ELISA, WB
- Conjugation: Biotin
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.45% Sodium chloride, 0.02% EDTA disodium salt, 0.27% Monobasic dihydrogen sodium phosphate, 0.73% Dibasic monohydrogen sodium phosphate
Concentration information loading...
Purification notesPurified by nitrated protein-Sepharose™
Our Abpromise guarantee covers the use of ab27646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Range 1/5000 - 1/40000. Predicted molecular weight: na kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceNitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
- NO tyrosine antibody
- nTyr antibody
ab27646 has been referenced in 2 publications.
- Ferreira I et al. Nitrated nucleosome levels and neuropsychiatric events in systemic lupus erythematosus; a multi-center retrospective case-control study. Arthritis Res Ther 19:287 (2017). PubMed: 29273092
- Croca S et al. Serum nitrated nucleosome levels in patients with systemic lupus erythematosus: a retrospective longitudinal cohort study. Arthritis Res Ther 16:R48 (2014). ELISA ; Human . PubMed: 24502558