Key features and details
- Goat polyclonal to Nitro tyrosine (FITC)
- Suitable for: ICC/IF
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Product nameAnti-Nitro tyrosine antibody (FITC)
See all Nitro tyrosine primary antibodies
DescriptionGoat polyclonal to Nitro tyrosine (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificitySpecifically binds to nitrated proteins. Molar F/P ratio is 4.6.
Tested applicationsSuitable for: ICC/IFmore details
Chemical/ Small Molecule (Nitrtyrosine modified protein)
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.2% PBS, 0.0146% EDTA, 0.435% Sodium chloride
Concentration information loading...
Purification notesPurified by nitrated protein-Sepharose™ affinity column.
Our Abpromise guarantee covers the use of ab27647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
RelevanceNitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
- NO tyrosine antibody
- nTyr antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab27647 has not yet been referenced specifically in any publications.