Anti-Nitro tyrosine antibody [HM.11] (ab7048)
- Datasheet
- Specific References (32)
- Protocols
Overview
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Product nameAnti-Nitro tyrosine antibody [HM.11]
See all Nitro tyrosine primary antibodies -
DescriptionMouse monoclonal [HM.11] to Nitro tyrosine
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Host speciesMouse
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SpecificityThis antibody reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The antibody does not recognize other antigens with a nitrotyrosine-like structure such as tyrosine, 3-chlorotyrosine, phosphotyrosine and phenylalanine.
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Tested applicationsSuitable for: WB, ICC/IF, ELISA, IHC-P, IHC-Frmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Nitro tyrosine (nitrated ) conjugated to Keyhole Limpet Haemocyanin (KLH).
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Positive control
- WB: mouse kidney lysate, mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate(see Abreviews) IHC-P: human lung tissue (see Abreview)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferConstituents: PBS, 0.1% BSA
WARNING!!: Do not add sodium azide or nitric containing perservative as it will damage the antibody. -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberHM.11
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IsotypeIgG2b
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab7048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 22462810 | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | 1/200 - 1/400. 10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100°C as antigen retrieval treatment | |
| IHC-Fr | Use at an assay dependent concentration. Acetone fixation 10 min -20°C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); block with 10% NGS or 5 % BSA for 30 min. |
Target
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RelevanceNitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
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Alternative names
- NO tyrosine antibody
- nTyr antibody
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Nitro tyrosine antibody [HM.11] (ab7048)](https://www.abcam.com/ps/products/7/ab7048/Images/Nitro-tyrosine-Primary-antibodies-ab7048-23.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nitro tyrosine antibody [HM.11] (ab7048)Image from Laschak M et al., BMC Cancer. 2012 Mar 30;12:130. Fig 1.; doi:10.1186/1471-2407-12-130; 30 March 2012, BMC Cancer 2012, 12:130Immunofluorescence analysis of 22Rv1 cells (Human prostate carcinoma epithelial cell line) treated with JS-K, staining Nitro tyrosine (green) with ab7048.
Cells were fixed with paraformaldehyde, permeabilized in 0.2% Triton X-100 and blocked with 1% BSA. Cells were incubated with primary antibody (1/25) for 30 min at 37°C. An AlexaFluor488®-conjugated anti-mouse IgG (1/100) was used as the secondary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nitro tyrosine antibody [HM.11] (ab7048)](https://www.abcam.com/ps/products/7/ab7048/Images/Nitro-tyrosine-Primary-antibodies-ab7048-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nitro tyrosine antibody [HM.11] (ab7048)ab7048 at 2 µg/ml staining Nitrotyrosine in Human liver from a severely obese patient. ab7048 was incubated for 1 hour at room temperature.
References
This product has been referenced in:
- Auger C et al. Metformin adapts its cellular effects to bioenergetic status in a model of metabolic dysfunction. Sci Rep 8:5646 (2018). Read more (PubMed: 29618839) »
- Zhang J et al. Repeated superovulation increases the risk of osteoporosis and cardiovascular diseases by accelerating ovarian aging in mice. Aging (Albany NY) 10:1089-1102 (2018). Read more (PubMed: 29787998) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"