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Anti-Nitro tyrosine antibody [HM.11] (ab7048)

Reviews (13)Q&A (21)References (64)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nitro tyrosine antibody [HM.11] (ab7048)

    Key features and details

    • Mouse monoclonal [HM.11] to Nitro tyrosine
    • Suitable for: IHC-Fr, IHC-P
    • Reacts with: Species independent
    • Isotype: IgG2b

    Conjugates logo Related conjugates and formulations

    Biotin

    Overview

    • Product name

      Anti-Nitro tyrosine antibody [HM.11]
      See all Nitro tyrosine primary antibodies

    • Description

      Mouse monoclonal [HM.11] to Nitro tyrosine

    • Host species

      Mouse

    • Specificity

      This antibody reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The antibody does not recognize other antigens with a nitrotyrosine-like structure such as tyrosine, 3-chlorotyrosine, phosphotyrosine and phenylalanine.

    • Tested applications

      Suitable for: IHC-Fr, IHC-Pmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Synthetic peptide corresponding to Nitro tyrosine (nitrated ) conjugated to Keyhole Limpet Haemocyanin (KLH).

    • Positive control

      • WB: mouse kidney lysate, mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate(see Abreviews) IHC-P: human lung tissue (see Abreview)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab7048 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr
    Use at an assay dependent concentration.

    Acetone fixation 10 min -20 degree C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); block with 10% NGS or 5 % BSA for 30 min.
    IHC-P (3)
    1/200 - 1/400. 10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100°C as antigen retrieval treatment
    Notes
    IHC-Fr
    Use at an assay dependent concentration.

    Acetone fixation 10 min -20 degree C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); block with 10% NGS or 5 % BSA for 30 min.
    IHC-P
    1/200 - 1/400. 10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100°C as antigen retrieval treatment

    Target

    • Relevance

      Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.

    • Alternative names

      • NO tyrosine antibody
      • nTyr antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nitro tyrosine antibody [HM.11] (ab7048)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nitro tyrosine antibody [HM.11] (ab7048)
      ab7048 at 2 µg/ml staining Nitrotyrosine in Human liver from a severely obese patient. ab7048 was incubated for 1 hour at room temperature.

    References (64)

    Publishing research using ab7048? Please let us know so that we can cite the reference in this datasheet.

    ab7048 has been referenced in 64 publications.

    • Liou GG  et al. N-Acetyl Cysteine Overdose Inducing Hepatic Steatosis and Systemic Inflammation in Both Propacetamol-Induced Hepatotoxic and Normal Mice. Antioxidants (Basel) 10:N/A (2021). PubMed: 33809388
    • Clayton ZS  et al. Apigenin restores endothelial function by ameliorating oxidative stress, reverses aortic stiffening, and mitigates vascular inflammation with aging. Am J Physiol Heart Circ Physiol 321:H185-H196 (2021). PubMed: 34114892
    • Qin X  et al. Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J mice†. Biol Reprod 104:1347-1359 (2021). PubMed: 33693648
    • Ramos H  et al. Beneficial Effects of Glucagon-Like Peptide-1 (GLP-1) in Diabetes-Induced Retinal Abnormalities: Involvement of Oxidative Stress. Antioxidants (Basel) 9:N/A (2020). PubMed: 32927585
    • Gioscia-Ryan RA  et al. Late-life voluntary wheel running reverses age-related aortic stiffness in mice: a translational model for studying mechanisms of exercise-mediated arterial de-stiffening. Geroscience N/A:N/A (2020). PubMed: 32529594
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    31-34 of 34 Abreviews or Q&A

    Question

    We are thinking to buy this antibody to use for ELISA on human plasma. I take look to your protocol for this application and I would prefer to use avidin-HRP for detection. Is that possible? Do you have the antibody link to streptavidin? With your 50ug format, for how many plate do I have enough? Do you sell QCs for this?

    Read More
    Answer

    Thank you for your enquiry and your interest in our product. We would like to confirm that this antibody has been tested and characterised for ELISA application. Each antibody and batch of conjugate should be titrated to determine the optimal working dilutions. For a general ELISA protocol, please click on the Resources section on the top panel of the Abcam homepage, you might find some useful information there. You can use ABC signal enhancing step. We do not have streptavidin-linked antibody against to this target. In order to apply this signal enhancing step, you need a secondary antibody which is biotin-labelled like ab:6788. Regarding the question - for how many plates you can use 50ug of antibody; the answer depends upon many factors: - the expressed levels of the target protein in your system, - dilution factor, - use if signal enhancing step (ABC), - detection system (HRP or ALP) - use of duplicates, triplicates or quadruplicates in each well etc. We hope this information will be useful.

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    Question

    I recently purchased this antibody from abcam in order to perform Western Blots (earlier this year I used it for immunohistochemistry and it worked fine). I would like to know the size of the product, ie what the molecular weight of nitrotyrosine is and where I should see the bands. The only reference I can find (Buttery et al, Lab Invest, 1996) describes bands between 180 and 30 kd which seems to be over a wide range. Do you know of any other paper that might suggest something more specific? Also if the technique for Westerns is described, it would help. Best wishes

    Read More
    Answer

    I am not an expert in this field, but would it not be the case that a number of proteins would contain tyrosine that has been nitrosylated and thus western blots using this antibody would reveal a wide range of bands? There are four references on the datasheet which may be of help.

    Read More

    Question

    We recently purchased ab7048, anti-nitrotyrosine monoclonal antibody from you. I wanted to know if it works well in rat?

    Read More
    Answer

    As far as I am aware, this antibody has been used extensively in human tissue, but has not yet been tested with rat.

    Read More

    Question

    One of the references in the datasheet for the Nitrotyrosine antibody 7048 (Ter Steege et al) is an ELISA method. Is this new antibody suitable for ELISA applications? ie. has the affinity of the antibody for nitrotyrosine residues in various proteins been tested? Thanks.

    Read More
    Answer

    The antibody IS suitable for ELISA applications. However, as the monoclonal antibody solution contains 0.1% BSA (decrease the stability of the antibody) this makes it unusable as coat antibody. This antibody does not recognize other antigens with a nitrotyrosine like structure such as tyrosine, 3-chlorotyrosine and phenylalanine, illustrating the specificity of the antibodies. This specificity of this antibody has been described in the mentioned article of Jessica ter Steege.

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    31-34 of 34 Abreviews or Q&A

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