Anti-Nitro tyrosine antibody [HM.11] (ab7048)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nitro tyrosine antibody [HM.11] (ab7048)](https://www.abcam.com/ps/products/7/ab7048/Images/ab7048-12600-anti-nitro-tyrosine-antibody-hm11-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nitro tyrosine antibody [HM.11] (ab7048)")
Key features and details
- Mouse monoclonal [HM.11] to Nitro tyrosine
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Species independent
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-Nitro tyrosine antibody [HM.11]
See all Nitro tyrosine primary antibodies -
Description
Mouse monoclonal [HM.11] to Nitro tyrosine -
Host species
Mouse -
Specificity
This antibody reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The antibody does not recognize other antigens with a nitrotyrosine-like structure such as tyrosine, 3-chlorotyrosine, phosphotyrosine and phenylalanine. -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to Nitro tyrosine (nitrated ) conjugated to Keyhole Limpet Haemocyanin (KLH).
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Positive control
- WB: mouse kidney lysate, mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate(see Abreviews) IHC-P: human lung tissue (see Abreview)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: PBS, 0.1% BSA
WARNING!!: Do not add sodium azide or nitric containing perservative as it will damage the antibody. -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HM.11 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
Acetone fixation 10 min -20 degree C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); block with 10% NGS or 5 % BSA for 30 min. |
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| IHC-P | (3) |
1/200 - 1/400. 10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100°C as antigen retrieval treatment
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| Notes |
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IHC-Fr
Use at an assay dependent concentration. Acetone fixation 10 min -20 degree C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); block with 10% NGS or 5 % BSA for 30 min. |
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IHC-P
1/200 - 1/400. 10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100°C as antigen retrieval treatment |
Target
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Relevance
Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques. -
Alternative names
- NO tyrosine antibody
- nTyr antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nitro tyrosine antibody [HM.11] (ab7048)ab7048 at 2 µg/ml staining Nitrotyrosine in Human liver from a severely obese patient. ab7048 was incubated for 1 hour at room temperature.
Datasheets and documents
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SDS download
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Datasheet download
References (64)
ab7048 has been referenced in 64 publications.
- Liou GG et al. N-Acetyl Cysteine Overdose Inducing Hepatic Steatosis and Systemic Inflammation in Both Propacetamol-Induced Hepatotoxic and Normal Mice. Antioxidants (Basel) 10:N/A (2021). PubMed: 33809388
- Clayton ZS et al. Apigenin restores endothelial function by ameliorating oxidative stress, reverses aortic stiffening, and mitigates vascular inflammation with aging. Am J Physiol Heart Circ Physiol 321:H185-H196 (2021). PubMed: 34114892
- Qin X et al. Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J mice†. Biol Reprod 104:1347-1359 (2021). PubMed: 33693648
- Ramos H et al. Beneficial Effects of Glucagon-Like Peptide-1 (GLP-1) in Diabetes-Induced Retinal Abnormalities: Involvement of Oxidative Stress. Antioxidants (Basel) 9:N/A (2020). PubMed: 32927585
- Gioscia-Ryan RA et al. Late-life voluntary wheel running reverses age-related aortic stiffness in mice: a translational model for studying mechanisms of exercise-mediated arterial de-stiffening. Geroscience N/A:N/A (2020). PubMed: 32529594