Application
Western blot
Sample
Human Tissue lysate - whole (Human Penile Tissue)
Loading amount
60 µg
Specification
Human Penile Tissue
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Starting Block T20 (PBS)
Secondary antibody
Name
Non-Abcam antibody was used: ECL Anti-mouse IgG
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/7000
Detection
Detection method
Chemiluminescence, ECL
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: ~50, 40, 25 kDa
Positive control
I did not have a positive control
Negative control
I did not have a negative control
Additional data
Additional Notes
I noticed strong bands around 50, 40 and 25 kDa. There were also weaker bands above 100 kDa. When I analyzed the blot I used the whole lane of bands from the top to the bottom.
Miss. Gwen Lagoda
Verified customer
Submitted Oct 19 2011