Key features and details
- Mouse monoclonal [HM.11] to Nitro tyrosine (Biotin)
- Suitable for: Other, WB, IHC-P, IHC-Fr
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2b
Product nameAnti-Nitro tyrosine antibody [HM.11] (Biotin)
See all Nitro tyrosine primary antibodies
DescriptionMouse monoclonal [HM.11] to Nitro tyrosine (Biotin)
SpecificityThis highly specific monoclonal antibody reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine.
Tested applicationsSuitable for: Other, WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Constituents: 0.164% Sodium phosphate, 1.45% Sodium chloride, 1.5% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab7225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Other||Use at an assay dependent concentration.|
|AP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|IHC-P||1/200 - 1/400.|
|IHC-Fr||Use at an assay dependent concentration.|
RelevanceNitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
- NO tyrosine antibody
- nTyr antibody
ab7225 has been referenced in 2 publications.
- Hathuc C et al. Nitration of human plasminogen by RAW 264.7 macrophages reduces streptokinase-induced plasmin activity. Clin Chem Lab Med 44:213-9 (2006). WB . PubMed: 16475910
- Bixby M et al. Ilex paraguariensis extracts are potent inhibitors of nitrosative stress: a comparative study with green tea and wines using a protein nitration model and mammalian cell cytotoxicity. Life Sci 77:345-58 (2005). WB . PubMed: 15878361