Anti-Nitro tyrosine antibody [HM.11] (Biotin) (ab7225)


  • Product name
    Anti-Nitro tyrosine antibody [HM.11] (Biotin)
    See all Nitro tyrosine primary antibodies
  • Description
    Mouse monoclonal [HM.11] to Nitro tyrosine (Biotin)
  • Host species
  • Conjugation
  • Specificity
    This highly specific monoclonal antibody reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat



Our Abpromise guarantee covers the use of ab7225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
AP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P 1/200 - 1/400.
IHC-Fr Use at an assay dependent dilution.


  • Relevance
    Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
  • Alternative names
    • NO tyrosine antibody
    • nTyr antibody


  • Nitrotyrosine in human liver of severely obese patients. Staining of paraffin tissue section with ab7225 at 2 μg/ml.


This product has been referenced in:
  • Hathuc C  et al. Nitration of human plasminogen by RAW 264.7 macrophages reduces streptokinase-induced plasmin activity. Clin Chem Lab Med 44:213-9 (2006). WB . Read more (PubMed: 16475910) »
  • Bixby M  et al. Ilex paraguariensis extracts are potent inhibitors of nitrosative stress: a comparative study with green tea and wines using a protein nitration model and mammalian cell cytotoxicity. Life Sci 77:345-58 (2005). WB . Read more (PubMed: 15878361) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for your inquiry. I would recommend to try a range of dilutions (1:1000- 1:20,000) for this antibody in Western blot. The optimal dilution needs to optimized according to the results.

These two references from the same lab suggest kidney carcinoma as a potential positive control for nitrotyrosine: Oxidative/nitrosative stress and peroxiredoxin 2 are associated with grade and prognosis of human renal carcinoma. APMIS. 2006 May;11...

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Thank you for your email. To use ab7225 in IHC, I would recommend titrating the antibody from 1:20 - 1:200, but you will have to optimize based upon your results. If you have any additional questions, please contact us again.

Thank you for your enquiry. I'm going to need your order number to tell you precisely how old the stock you received is. To use ab7225 in IHC, I would recommend starting at a dilution of 1:10, but you should try a range in order to optimize the best re...

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Thank you for your enquiry. The following article is a reference for ab7225: 1) Jessica Ter Steege et al: Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich Elisa: Free Radiac boil Med 25 (8): 9543-963, 1998. For...

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This antibody has been found to work well in formalin fixed paraffin embedded tissue and is expected to recognise nitrotyrosine in all species. Heat mediated antigen retrieval will probably be beneficial. Please refer to the Antigen Retrieval protocol,...

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