• Product name

    Anti-Nitro tyrosine antibody (HRP)
    See all Nitro tyrosine primary antibodies
  • Description

    Goat polyclonal to Nitro tyrosine (HRP)
  • Host species

  • Conjugation

  • Tested applications

    Suitable for: WB, ELISAmore details
  • Immunogen

    Chemical/ Small Molecule (nitrotyrosine modified protein)



Our Abpromise guarantee covers the use of ab27648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: 1/1000 - 1/4000.
    WB: 1/1000 - 1/4000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
    • Alternative names

      • NO tyrosine antibody
      • nTyr antibody


    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A


    Thank you for contacting us and reporting the problems you have encountered with the Anti-Nitro tyrosine antibody (HRP) lot number 874384. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. As we discussed on the phone, I have checked that the product concentration has not altered between the lots used (both supplied at 1 mg/ml), you should therefore not be seeing a diminished signal due to this. As I mentioned on the phone, it may be that this particular batch is unfortunately faulty. This does occur occasionally but I am very sorry for any inconvenience caused by this. You mentioned in our phone conversation that the ELISA being used is for diagnostic purposes. Abcam is a life science supplier of research reagents only. All products supplied by Abcam are not intended for diagnostic or therapeutic use. Our products are not produced to the higher quality control standards required for such applications. Furthermore, Abcam will not take responsibility or pay compensation for time or business lost as our products are intended for research and development purpose only. If you have any question regarding this policy please let me know. Alternatively more information can be found from the following link: https://www.abcam.com/index.html?pageconfig=terms

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 977603. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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