Anti-Nitro tyrosine (biotinylated ) [8C7.3] antibody (ab24496)

Overview

  • Product name
    Anti-Nitro tyrosine (biotinylated ) [8C7.3] antibody
    See all Nitro tyrosine primary antibodies
  • Description
    Mouse monoclonal [8C7.3] to Nitro tyrosine (biotinylated )
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Full length native protein (purified) corresponding to Nitro tyrosine (biotinylated ).

Properties

Applications

Our Abpromise guarantee covers the use of ab24496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques.
  • Alternative names
    • NO tyrosine antibody
    • nTyr antibody

References

This product has been referenced in:
  • Alhalwani AY  et al. Development of a sandwich ELISA with potential for selective quantification of human lactoferrin protein nitrated through disease or environmental exposure. Anal Bioanal Chem 410:1389-1396 (2018). Read more (PubMed: 29214534) »
  • Pais TF  et al. The NAD-dependent deacetylase sirtuin 2 is a suppressor of microglial activation and brain inflammation. EMBO J 32:2603-16 (2013). IHC-P ; Mouse . Read more (PubMed: 24013120) »

See all 2 Publications for this product

Customer reviews and Q&As

Using a mouse antibody to for mouse IHC can lead to high background if you are detecting the antibody with a secondary anti-mouse IgG antibody, since the anti-IgG may detect endogenous IgG in the sample. You can check for that by incubating a section w...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount
30 µg
Specification
Melanoma cancer
Treatment
5gy Irradiation
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

Submitted Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Melanoma cancer)
Specification
Melanoma cancer
Fixative
4% Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 121 Celsius for 10min autoclaved
Permeabilization
Yes - 0.1% SDS
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

Submitted Dec 20 2012

Thank you for getting back to me and for answering to my further questions promptly.

I have been discussing this enquiry with my colleague in the Lab and the methods seem to be chosen all look appropriate ECl, milk block etc, Xray film.
...

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I am very sorry to hear that both antibodies did not work properly (ab24496 and ab110282).

I can offer you a replacement vial as you wish but it would be important to find out why two antibodies failed to work.

I have read through a...

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Thank you for your enquiry. It is very unfortunate that both of these products (ab24496 and ab110282) do not perform as they are expected to do so.

I would like to reassure you that I take your comment seriously. Though, you have kindly provi...

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Thank you for your response. This is to let you know that I have placed a new order for you - for one vial of ab110282 as a free of charge replacement (exchange for the original item ab24496) and the new order number is 992370. I hope the s...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. Looking at the WB image you have kindly attached, it ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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