Biotin Anti-Nitrotyrosine antibody [8C7.3] (ab24496)
Key features and details
- Biotin Mouse monoclonal [8C7.3] to Nitrotyrosine
- Suitable for: IHC-P, WB, ELISA, IP, IHC-Fr
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG2b
Overview
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Product name
Biotin Anti-Nitrotyrosine antibody [8C7.3]
See all Nitrotyrosine primary antibodies -
Description
Biotin Mouse monoclonal [8C7.3] to Nitrotyrosine -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-P, WB, ELISA, IP, IHC-Frmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length native protein (purified) corresponding to Nitro tyrosine (biotinylated ).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
8C7.3 -
Isotype
IgG2b -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 1 µg/ml.
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. |
ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Relevance
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab24496 has been referenced in 2 publications.
- Alhalwani AY et al. Development of a sandwich ELISA with potential for selective quantification of human lactoferrin protein nitrated through disease or environmental exposure. Anal Bioanal Chem 410:1389-1396 (2018). PubMed: 29214534
- Pais TF et al. The NAD-dependent deacetylase sirtuin 2 is a suppressor of microglial activation and brain inflammation. EMBO J 32:2603-16 (2013). IHC-P ; Mouse . PubMed: 24013120