Nitrocefin, Chromogenic cephalosporin beta-lactamase substrate (ab145625)

Overview

  • Product name

    Nitrocefin, Chromogenic cephalosporin beta-lactamase substrate
  • Description

    Chromogenic cephalosporin β-lactamase substrate
  • Biological description

    Chromogenic cephalosporin β-lactamase substrate. Indicates β-lactamase activity via a red color intensely proportional to the original concentration of Nitrocefin. Undergoes rapid distinctive color change from yellow (max at pH 7.0 = 390 nm) to red (max at pH 7.0 = 486 nm) as the amide bond in the beta-lactam ring is hydrolyzed.
  • Purity

    > 95%
  • CAS Number

    41906-86-9
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    (6R,7R)-3-[(E)-2-(2,4-Dinitrophenyl)ethenyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
  • Molecular weight

    516.50
  • Molecular formula

    C21H16N4O8S2
  • PubChem identifier

    6436140
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 50 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    C1C(=C(N2[C@H](S1)[C@@H](C2=O)NC(=O)CC3=CC=CS3)C(=O)O)/C=C/C4=C(C=C(C=C4)[N+](=O)[O-])[N+](=O)[O-]
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Buyck JM  et al. Increased susceptibility of Pseudomonas aeruginosa to macrolides and ketolides in eukaryotic cell culture media and biological fluids due to decreased expression of oprM and increased outer-membrane permeability. Clin Infect Dis 55:534-42 (2012). Read more (PubMed: 22573850) »
  • Mori N  et al. A peptide based on homologous sequences of the ß-barrel assembly machinery component BamD potentiates antibiotic susceptibility of Pseudomonas aeruginosa. J Antimicrob Chemother 67:2173-81 (2012). Read more (PubMed: 22628248) »
  • Uri JV Detection of beta-lactamase activity with nitrocefin of multiple strains of various microbial genera. Acta Microbiol Hung 32:133-45 (1985). Read more (PubMed: 3933272) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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