- References (1)
Product nameNitrotetrazolium blue chloride (NBT), Chromogenic substrate
- Nitro BT
- Nitro-Blue tetrazolium chloride
- NSC 27622
Chromogenic substrate for dehydrogenases and other oxidases that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. Acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).
Chemical name3,3'-[3,3'-Dimethoxy(1,1'-biphenyl)-4,4'-diyl]bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium dichloride
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Trinh le A et al. Fluorescent in situ hybridization employing the conventional NBT/BCIP chromogenic stain. Biotechniques 42:756-9 (2007). Read more (PubMed: 17612300) »
- Nisimoto Y et al. NADH dehydrogenase from bovine neutrophil membranes. Purification and properties. J Biol Chem 261:285-90 (1986). Read more (PubMed: 3941077) »
- Freeman R & King B Technique for the performance of the nitro-blue tetrazolium (NBT) test. J Clin Pathol 25:912-4 (1972). Read more (PubMed: 4119008) »