Key features and details
- Mouse monoclonal [39B6] to Nitrotyrosine (Biotin)
- Suitable for: WB, ICC/IF, IHC-Fr, IP, IHC-P
- Reacts with: Mouse, Rat, Dog, Human
- Conjugation: Biotin
- Isotype: IgG2a
Product nameAnti-Nitrotyrosine antibody [39B6] (Biotin)
See all Nitrotyrosine primary antibodies
DescriptionMouse monoclonal [39B6] to Nitrotyrosine (Biotin)
Specificityab183392 recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human
Chemical/ Small Molecule corresponding to Nitrotyrosine conjugated to Bovine Serum Albumin (BSA). 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid-BSA conjugate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab183392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.7 µg/ml.
0.7μg/ml was sufficient for detection of 5µg SIN-1 treated BSA by Western Blot using Goat anti-mouse IgG (HRP) as the secondary antibody.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
RelevanceThe cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
ab183392 has not yet been referenced specifically in any publications.