Overview

  • Product name

    Anti-Nitrotyrosine antibody [39B6] (Biotin)
    See all Nitrotyrosine primary antibodies
  • Description

    Mouse monoclonal [39B6] to Nitrotyrosine (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Specificity

    ab183392 recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Fr, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Chemical/ Small Molecule corresponding to Nitrotyrosine conjugated to Bovine Serum Albumin (BSA). 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid-BSA conjugate.

Properties

Applications

Our Abpromise guarantee covers the use of ab183392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.7 µg/ml.

0.7μg/ml was sufficient for detection of 5µg SIN-1 treated BSA by Western Blot using Goat anti-mouse IgG (HRP) as the secondary antibody.

ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

References

ab183392 has not yet been referenced specifically in any publications.

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