Key features and details
- Mouse monoclonal [39B6] to Nitrotyrosine (FITC)
- Suitable for: WB, IHC-Fr, ICC/IF, IP, IHC-P
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Product nameAnti-Nitrotyrosine antibody [39B6] (FITC)
See all Nitrotyrosine primary antibodies
DescriptionMouse monoclonal [39B6] to Nitrotyrosine (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Specificityab183390 recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
Tested applicationsSuitable for: WB, IHC-Fr, ICC/IF, IP, IHC-Pmore details
Chemical/ Small Molecule corresponding to Nitrotyrosine conjugated to Bovine Serum Albumin (BSA). 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid-BSA conjugate.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab183390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.7 µg/ml.
0.7μg/ml of ab183390 was sufficient for detection of 5μg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG (HRP) as the secondary antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
RelevanceThe cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
ab183390 has not yet been referenced specifically in any publications.