Overview

  • Product name

    Anti-Nitrotyrosine antibody [39B6] (HRP)
    See all Nitrotyrosine primary antibodies
  • Description

    Mouse monoclonal [39B6] to Nitrotyrosine (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Chemical/ Small Molecule corresponding to Nitrotyrosine conjugated to Bovine Serum Albumin (BSA). 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid-BSA conjugate.

Properties

Applications

Our Abpromise guarantee covers the use of ab183391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.7 µg/ml.

0.7μg/ml was sufficient for detection of 5µg SIN-1 treated BSA by Western Blot using Goat anti-mouse IgG (HRP) as the secondary antibody.

IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

References

This product has been referenced in:

  • Jing W  et al. Uremia induces upregulation of cerebral tissue oxidative/inflammatory cascade, down-regulation of Nrf2 pathway and disruption of blood brain barrier. Am J Transl Res 10:2137-2147 (2018). Read more (PubMed: 30093950) »
  • Jing W  et al. LCZ696 (Sacubitril/valsartan) ameliorates oxidative stress, inflammation, fibrosis and improves renal function beyond angiotensin receptor blockade in CKD. Am J Transl Res 9:5473-5484 (2017). WB ; Rat . Read more (PubMed: 29312499) »
See all 2 Publications for this product

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