Overview

  • Product name
    Anti-Nitrotyrosine antibody [EM-30]
    See all Nitrotyrosine primary antibodies
  • Description
    Mouse monoclonal [EM-30] to Nitrotyrosine
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Nitrotyrosine.

  • Positive control
    • NO2-BSA, NO2-HSA; Human colon cancer epithelium and prostate hyperplasia tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab125106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.

Target

  • Relevance
    The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

Images

  • Immunohistochemical analysis of Human tumoral colon epithelium (frozen sections) labelling Nitrotyrosine with ab125106 at 10 µg/mL. 

References

This product has been referenced in:
  • Ogunbileje JO  et al. Hypermetabolism and hypercatabolism of skeletal muscle accompany mitochondrial stress following severe burn trauma. Am J Physiol Endocrinol Metab 311:E436-48 (2016). Read more (PubMed: 27382037) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your reply.

To confirm, the epitope for this antibody is not species specific. In theory, it should detect Nitrotyrosine in all species. However, I have collected the following information for you:

ab125106 Anti-Nitrotyrosine antibody [EM-30]
This antibody was tested on human and bovine samples.

ab78163 Anti-Nitrotyrosine antibody [2A8.2]
I can confirm this antibody has been tested in IHC on mouse brain tissue sections.
The following publications cited the use of this product on various species:

Cheng, C.Y., et al. 2010. Acta Pharmacol. Sinica 31:889-899 (rat; by IHC, Fig 2 - 4)

Kupai, K., et al. 2009. Am. J. Phsysiol. Heart Circ. Physiol. 297(5):H1729-H1735 (rat; by ELISA, Fig. 4)

Rai, S., et al. 2009. Int. J. Exp. Pathol. 90(6):598-604 (Rabbit; by IHC, Fig. 2)

Kiss, A., et al. 2008. Br. J. Pharmacol. 155(7):1015-1024 (dog; by Western blotting, Fig. 6)

Takahashi, Y., et al. 2007. Gastroenterol. 132(3):938-943 (Human; by IHC, Similar as 8OHdG staining in Fig. 3 as mentioned in the Results section)

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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