For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Customer has contacted us as their samples are giving a "reverse" reading. Those with the highest 3NT are giving the highest response. There are using human plasma and are preparing it by diluting to a 1mg/ml solution and then performing a series dilution on this 1mg/ml sample to test.
Asked on Sep 06 2012
Thank you for contacting us.
I have been in touch with the lab regarding your issue.Unfortunately we are at a loss to explain why more protein would cause a greater signal. There is, as we spoke of on the phone, the possibility that the high proteins concentrations could be making the antibody crash and stick to the plate but that’s unlikely. 1 mg/mL is a lot of protein sample. It may be an issue of interference causing non-specifically binding and low signal. We wouldcertainly recommend using a lower concentration sample to begin.
If you could send us your data and any other information regarding this issue, I would be more than happy to review it with the lab.
This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
Answered on Sep 06 2012