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Thank you very much for your time and help! I’m, however, a little confused in that the higher protein concentration samples confounded with the assay and lower protein samples didn’t have enough nitration to be detectable. To your knowledge, have anybody else measured 3NT levels in human plasma samples successfully? The samples I tried was from a healthy adolescent and a kid with type I diabetics. Would they be different from adult samples from the experience you have? Sorry for all these questions but I really wish the assay works.
Asked on Sep 10 2012
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We haven’t measured nitration in serum. Our lab wants to make sure that when using this kitthat the blocking buffer, diluents, pH and ionic strength for all samples and standards was thepreciselythe same.If we can seeexactlyhow the samples were prepared that will help. Could you please send us the detailed steps you have taken so that we may better understand this problem?
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Answered on Sep 10 2012