Key features and details
- Sensitivity: 10 ng/ml
- Range: 30 ng/ml - 3000 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameNivolumab ELISA Kit
See all Nivolumab kits
Intra-assay Sample n Mean SD CV% Overall < 15% Inter-assay Sample n Mean SD CV% Overall < 15%
Sample typeSerum, Plasma
Assay typeSandwich (quantitative)
Range30 ng/ml - 3000 ng/ml
Sample specific recovery Sample type Average % Range Serum 85% - 115%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Note: Nivolumab ELISA kit ab237651 is intended for Research Use Only, not for use in diagnostic procedures.
This kit is a sandwich based ELISA kit. The color developed is proportional to the amount of Nivolumab in the sample or standard. Results of samples can be determined directly using the standard curve.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 96 tests Micro ELISA Plate 1 unit Nivolumab Standard S1 1 x 0.3ml Nivolumab Standard S2 1 x 0.3ml Nivolumab Standard S3 1 x 0.3ml Nivolumab Standard S4 1 x 0.3ml Nivolumab Standard S5 1 x 0.3ml Nivolumab Standard S6 1 x 0.3ml Nivolumab Standard S7 1 x 0.3ml Nivolumab Standard S8 1 x 0.3ml Assay Buffer 2 x 50ml HRP-conjugate Probe 1 x 12ml TMB substrate 1 x 12ml Stop Solution 1 x 12ml Wash buffer (20X) 1 x 50ml Plate sealers 2 units
RelevanceNivolumab is a human immunoglobulin G4 (IgG4) monoclonal antibody that binds to the programmed cell death 1 (PD-1) receptor and selectively blocks interaction with its programmed death ligands PD-L1 and PD-L2. Upregulation of PD-1 ligands occurs in some tumors and signalling through this pathway can contribute to inhibition of active T-cell immune surveillance of tumour tissue. The inhibitory effect of PD-1 and its ligands occurs through the promotion of apoptosis in antigen specific T cells while simultaneously blocking apoptosis in suppressor T cells. Blocking PD-1 activity has been shown to lead to decreased tumour growth in mouse tumour models.
ab237651 has not yet been referenced specifically in any publications.