Overview

  • Product name

    Nivolumab ELISA Kit (Opdivo®)
    See all Nivolumab kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 15%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 15%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    10 ng/ml
  • Range

    30 ng/ml - 3000 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85% - 115%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Nivolumab ELISA Kit (Opdivo®) (ab237651) has been developed for specific quantification of Nivolumab concentration in human serum or plasma with high sensitivity and reproducibility. The density of color is proportional to the amount of Omalizumab captured from the samples and can be quantified when compared with standard curve.


    Nivolumab (Opdivo®) is a human immunoglobulin G4 (IgG4) monoclonal antibody that binds to the programmed cell death 1 (PD-1) receptor and selectively blocks interaction with its programmed death ligands PD-L1 and PD-L2. Upregulation of PD-1 ligands occurs in some tumors and signalling through this pathway can contribute to inhibition of active T-cell immune surveillance of tumour tissue. The inhibitory effect of PD-1 and its ligands occurs through the promotion of apoptosis in antigen specific T cells while simultaneously blocking apoptosis in suppressor T cells. Blocking PD-1 activity has been shown to lead to decreased tumour growth in mouse tumour models.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 96 tests
    Micro ELISA Plate 1 unit
    Nivolumab Standard S1 1 x 0.3ml
    Nivolumab Standard S2 1 x 0.3ml
    Nivolumab Standard S3 1 x 0.3ml
    Nivolumab Standard S4 1 x 0.3ml
    Nivolumab Standard S5 1 x 0.3ml
    Nivolumab Standard S6 1 x 0.3ml
    Nivolumab Standard S7 1 x 0.3ml
    Nivolumab Standard S8 1 x 0.3ml
    Assay Buffer 2 x 50ml
    HRP-conjugate Probe 1 x 12ml
    TMB substrate 1 x 12ml
    Stop Solution 1 x 12ml
    Wash buffer (20X) 1 x 50ml
    Plate sealers 2 units
  • Relevance

    Nivolumab (Opdivo®) is a human immunoglobulin G4 (IgG4) monoclonal antibody that binds to the programmed cell death 1 (PD-1) receptor and selectively blocks interaction with its programmed death ligands PD-L1 and PD-L2. Upregulation of PD-1 ligands occurs in some tumors and signalling through this pathway can contribute to inhibition of active T-cell immune surveillance of tumour tissue. The inhibitory effect of PD-1 and its ligands occurs through the promotion of apoptosis in antigen specific T cells while simultaneously blocking apoptosis in suppressor T cells. Blocking PD-1 activity has been shown to lead to decreased tumour growth in mouse tumour models.
  • Alternative names

    • Opdivo

Applications

Our Abpromise guarantee covers the use of ab237651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.

Protocols

References

ab237651 has not yet been referenced specifically in any publications.

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