Product nameAnti-NK-1R antibody [EPR6836(2)]
See all NK-1R primary antibodies
DescriptionRabbit monoclonal [EPR6836(2)] to NK-1R
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
within Human NK-1R aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: P25103
- K562, Jurkat and THP-1 cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab183713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/50 - 1/70.|
|WB||1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).|
FunctionThis is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- Neurokinin 1 antibody
- Neurokinin 1 Receptor antibody
- NK 1 receptor antibody
Western blot analysis of NK-1R in K562 cell lysate immunoprecipitated using ab183713 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of K562 lysates.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
All lanes : Anti-NK-1R antibody [EPR6836(2)] (ab183713) at 1/50000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 46 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Muschter D et al. Sensory Neuropeptides and their Receptors Participate in Mechano-Regulation of Murine Macrophages. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30682804) »
- He ZX et al. Substance P plays a critical role in synaptic transmission in striatal neurons. Biochem Biophys Res Commun 511:369-373 (2019). Read more (PubMed: 30803756) »