Recombinant
RabMAb

Recombinant Anti-NK-1R antibody [EPR6836(2)] - BSA and Azide free (ab232653)

Overview

  • Product name

    Anti-NK-1R antibody [EPR6836(2)] - BSA and Azide free
    See all NK-1R primary antibodies
  • Description

    Rabbit monoclonal [EPR6836(2)] to NK-1R - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NK-1R aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: P25103

  • Positive control

    • WB: K562, Jurkat and THP-1 cell lysates.
  • General notes

    Ab232653 is the carrier-free version of ab183713. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6836(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Neurokinin 1 antibody
    • Neurokinin 1 Receptor antibody
    • NK 1 receptor antibody
    • NK 1R antibody
    • NK-1 receptor antibody
    • NK-1R antibody
    • NK1 receptor antibody
    • NK1R antibody
    • NK1R_HUMAN antibody
    • NKIR antibody
    • SPR antibody
    • Substance P receptor antibody
    • Substance-P receptor antibody
    • TAC 1R antibody
    • TAC1R antibody
    • Tachykinin 1 receptor (substance P receptor neurokinin 1 receptor) antibody
    • Tachykinin receptor 1 (substance P receptor neurokinin 1 receptor) antibody
    • Tachykinin receptor 1 antibody
    • TACR1 antibody
    see all

Images

  • Western blot analysis of NK-1R in K562 cell lysate immunoprecipitated using ab183713 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of K562 lysates.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183713).

  • All lanes : Anti-NK-1R antibody [EPR6836(2)] (ab183713) at 1/50000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 3 : THP-1 (human monocytic leukemia cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183713).

References

ab232653 has not yet been referenced specifically in any publications.

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