Anti-NK-2R antibody (ab233295)
Key features and details
- Rabbit polyclonal to NK-2R
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-NK-2R antibody
See all NK-2R primary antibodies -
Description
Rabbit polyclonal to NK-2R -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-tag) corresponding to Human NK-2R aa 50-300. Expressed in E.coli. N-terminal tag.
Database link: P21452 -
Positive control
- WB: Human lung lysate; HeLa cell lysate; Pig small intestine and heart lysate; Recombinant human NK-2R protein. IHC-P: Human pancreatic cancer, breast cancer and stomach tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml.
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. |
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
This is a receptor for the tachykinin neuropeptide substance K (neurokinin A). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance K > neuromedin-K > substance P. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6865 Human
- Omim: 162321 Human
- SwissProt: P21452 Human
- Unigene: 88372 Human
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Alternative names
- Neurokinin 2 receptor antibody
- Neurokinin A receptor antibody
- NK 2 receptor antibody
see all
Images
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Formalin-fixed, paraffin-embedded human stomach tissue stained for NK-2R using ab233295 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NK-2R using ab233295 (green) at 10 µg/mL in ICC/IF. FITC-labeled secondary antibody.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for NK-2R using ab233295 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for NK-2R using ab233295 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Anti-NK-2R antibody (ab233295) at 1 µg/ml + Pig heart lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml -
Anti-NK-2R antibody (ab233295) at 1 µg/ml + Pig small intestine lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml -
Anti-NK-2R antibody (ab233295) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml -
Anti-NK-2R antibody (ab233295) at 1 µg/ml + Human lung lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml -
Anti-NK-2R antibody (ab233295) at 2 µg/ml + Recombinant human NK-2R protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233295 has not yet been referenced specifically in any publications.