Anti-NK-3R antibody (ab124025)
Key features and details
- Rabbit polyclonal to NK-3R
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NK-3R antibody -
Description
Rabbit polyclonal to NK-3R -
Host species
Rabbit -
Specificity
ab124025 does not recognise NK1R or NK2R. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human NK-3R (internal sequence).
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Positive control
- Human brain tissue; RAW 264.7 whole cell lysate
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General notes
This product should be stored undiluted.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 52 kDa). |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
This is a receptor for the tachykinin neuropeptide neuromedin-K (neurokinin B). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: neuromedin-K > substance K > substance P. -
Involvement in disease
Hypogonadotropic hypogonadism 11 with or without anosmia -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsThe anchoring of this receptor to the plasma membrane is probably mediated by the palmitoylation of a cysteine residue. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6870 Human
- Entrez Gene: 21338 Mouse
- Omim: 162332 Human
- SwissProt: P29371 Human
- SwissProt: P47937 Mouse
- Unigene: 942 Human
- Unigene: 103810 Mouse
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Alternative names
- MGC148060 antibody
- MGC148061 antibody
- Neurokinin B receptor antibody
see all
Images
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Lane 1 : Anti-NK-3R antibody (ab124025) at 0.5 µg/ml
Lane 2 : Anti-NK-3R antibody (ab124025) at 2 µg/ml
All lanes : RAW 264.7 whole cell lysate
Predicted band size: 52 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted? -
ab124025, at 5µg/ml, staining NK-3R in paraffin-embedded Human brain tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab124025 has been referenced in 1 publication.
- Vielma AH & Schmachtenberg O Electrophysiological fingerprints of OFF bipolar cells in rat retina. Sci Rep 6:30259 (2016). IHC-P ; Rat . PubMed: 27457753