Key features and details
- Rabbit polyclonal to NK-3R
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NK-3R antibody
See all NK-3R primary antibodies
DescriptionRabbit polyclonal to NK-3R
Specificityab124025 does not recognise NK1R or NK2R.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human NK-3R (internal sequence).
(Peptide available as
- Human brain tissue; RAW 264.7 whole cell lysate
Protein previously labeled as Neurokinin 3 Receptor NK3.This product should be stored undiluted.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab124025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 52 kDa).Can be blocked with NK-3R peptide (ab176411).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThis is a receptor for the tachykinin neuropeptide neuromedin-K (neurokinin B). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: neuromedin-K > substance K > substance P.
Involvement in diseaseHypogonadotropic hypogonadism 11 with or without anosmia
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsThe anchoring of this receptor to the plasma membrane is probably mediated by the palmitoylation of a cysteine residue.
Cellular localizationCell membrane.
- Information by UniProt
- MGC148060 antibody
- MGC148061 antibody
- Neurokinin B receptor antibody
Lane 1 : Anti-NK-3R antibody (ab124025) at 0.5 µg/ml
Lane 2 : Anti-NK-3R antibody (ab124025) at 2 µg/ml
All lanes : RAW 264.7 whole cell lysate
Predicted band size: 52 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
ab124025, at 5µg/ml, staining NK-3R in paraffin-embedded Human brain tissue by Immunohistochemistry.
ab124025 has been referenced in 1 publication.
- Vielma AH & Schmachtenberg O Electrophysiological fingerprints of OFF bipolar cells in rat retina. Sci Rep 6:30259 (2016). IHC-P ; Rat . PubMed: 27457753