Overview

  • Product name

  • Description

    Rabbit polyclonal to NK-3R
  • Host species

    Rabbit
  • Specificity

    ab124025 does not recognise NK1R or NK2R.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human NK-3R (internal sequence).
    (Peptide available as ab176411)

  • Positive control

    • Human brain tissue; RAW 264.7 whole cell lysate
  • General notes

    Protein previously labeled as Neurokinin 3 Receptor NK3.

    This product should be stored undiluted.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 52 kDa).Can be blocked with NK-3R peptide (ab176411).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    This is a receptor for the tachykinin neuropeptide neuromedin-K (neurokinin B). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: neuromedin-K > substance K > substance P.
  • Involvement in disease

    Hypogonadotropic hypogonadism 11 with or without anosmia
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    The anchoring of this receptor to the plasma membrane is probably mediated by the palmitoylation of a cysteine residue.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC148060 antibody
    • MGC148061 antibody
    • Neurokinin B receptor antibody
    • Neurokinin beta receptor antibody
    • Neuromedin K Receptor antibody
    • Neuromedin-K receptor antibody
    • NK 3 receptor antibody
    • NK 3R antibody
    • NK-3 receptor antibody
    • NK-3R antibody
    • NK3 receptor antibody
    • NK3R antibody
    • NK3R_HUMAN antibody
    • NKR antibody
    • TAC 3R antibody
    • TAC3R antibody
    • TAC3RL antibody
    • Tachykinin receptor 3 antibody
    • TACR 3 antibody
    • Tacr3 antibody
    see all

Images

  • Lane 1 : Anti-NK-3R antibody (ab124025) at 0.5 µg/ml
    Lane 2 : Anti-NK-3R antibody (ab124025) at 2 µg/ml

    All lanes : RAW 264.7 whole cell lysate

    Predicted band size: 52 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?

  • ab124025, at 5µg/ml, staining NK-3R in paraffin-embedded Human brain tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Vielma AH & Schmachtenberg O Electrophysiological fingerprints of OFF bipolar cells in rat retina. Sci Rep 6:30259 (2016). IHC-P ; Rat . Read more (PubMed: 27457753) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Nasal mucosa)
Antigen retrieval step
None
Permeabilization
No
Specification
Nasal mucosa
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5 · Temperature: RT°C
Fixative
Zamboni

Mr. Duc Dung Le

Verified customer

Submitted Apr 29 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Specification
Brain
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Duc Dung Le

Verified customer

Submitted Apr 29 2015

Answer

Thank you for taking the time to complete our questionnaires and contact us. I am sorry to hearthe customer has had difficulty obtaining satisfactory results from these antibodies.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

To confirm with you, four antibodies are mentioned in your email. However, 2 copies of the questionnaire for ab124025 have been provided and there is no questionnaire for ab11259.

I can provide a response for ab94585,ab124025and ab31928. If you require further help regarding ab11529, please do not hesitate to send the questionnaire and I will be pleased to help.

I would like to reassure you that ab94585 and ab31928 are tested and covered by our 6 month guarantee for use in IHC-Fr and mouse samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement. Regarding ab124025, this is tested and guaranteed in mouse, but has not been tested in frozen sections. Could you confirm if the customer has tried paraffin sections, for which we can provide the guarantee?

Reviewing this case, I would like to offer some suggestions to help optimize the results from ab94585,ab124025and ab31928. I would also appreciate if you can confirm some further details. There are similar comments to provide for all three antibodies:

1. Fixation 1 hour for frozen sections is a very long time. For Frozen sections, we recommend fixation for 10 minutes only. Otherwize there may be too much protein crosslinking from the PFA, and the epitope may not be accessible to the antibody.

2. As far as I am aware, Recoverin, GABA A Receptor alpha 1 and Neurokinin 3 Receptor NK3 are all membrane or membraneassociated protein.Detection of thesewill not require permeabilization. Indeed, Triton is a strong detergent and can disrupt membrane proteins and eptiopes, which can significantly affect the staining. Therefore, I can suggest to remove the permeabilization step.

3. I can recommend to use a more gentle detergent, such as Tween 20 in the other buffers, try 0.1% - 0.2% Tween.

4. I recommend to include 0.1 - 0.2% tween in the wash buffer, this will help to wash away any excess antibody.

5. We recommend not to mix blocking agents in an experiment. try serum only.

6. Could you confirm, are the current vials of secondary antibodies working well with other primary antibodies? What were the results of the no primary controls? The concentration of secondary antibody may need to be reduced to help optimize the results.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details, including information regarding ab11259.

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