RecombinantRabMAb

Recombinant Anti-NK-p44 antibody [EPR23732-129] (ab259346)

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Flow Cytometry - Anti-NK-p44 antibody [EPR23732-129] (ab259346)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Rabbit monoclonal [EPR23732-129] to NK-p44
    • Suitable for: Flow Cyt
    • Reacts with: Human

    Overview

    • Product name

      Anti-NK-p44 antibody [EPR23732-129]
      See all NK-p44 primary antibodies

    • Description

      Rabbit monoclonal [EPR23732-129] to NK-p44

    • Host species

      Rabbit

    • Tested applications

      Suitable for: Flow Cytmore details
      Unsuitable for: ICC,IHC-P,IP or WB

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • Flow Cyt: Human peripheral blood mononuclear cell (PBMC) treated with 20ng/ml Interleukin-2 (IL-2) for 7 days

    • General notes

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
    • Concentration information loading...

    • Purity

      Protein A purified

    • Clonality

      Monoclonal

    • Clone number

      EPR23732-129

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab259346 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    1/500.
    Notes
    Flow Cyt
    1/500.
  • Application notes
    Is unsuitable for ICC,IHC-P,IP or WB.

    • Target

    • Function

      Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

    • Tissue specificity

      Selectively expressed by activated NK cells and by in vitro cultured (i.e. activated) TCRg/d lymphoid cells.

    • Sequence similarities

      Belongs to the natural cytotoxicity receptor (NCR) family.
      Contains 1 Ig-like (immunoglobulin-like) domain.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Activating NK receptor NKp44 antibody
        • CD336 antibody
        • CD336 antigen antibody
        • dJ149M18.1 antibody
        • LY95 antibody
        • Lymphocyte antigen 95 (activating NK receptor; NK p44) antibody
        • Lymphocyte antigen 95 homolog (activating NK receptor; NK p44) antibody
        • Lymphocyte antigen 95 homolog antibody
        • Lymphocyte antigen 95, mouse, homolog of antibody
        • Natural cytotoxicity triggering receptor 2 antibody
        • Natural killer cell p44 related protein antibody
        • Natural killer cell p44-related protein antibody
        • NCR2 antibody
        • NCTR2_HUMAN antibody
        • NK cell activating receptor (NKp44) antibody
        • NK cell activating receptor antibody
        • NK cell-activating receptor antibody
        • NK p44 antibody
        • NK-p44 antibody
        • NKp44 antibody
        see all

      Images

      • Flow Cytometry - Anti-NK-p44 antibody [EPR23732-129] (ab259346)
        Flow Cytometry - Anti-NK-p44 antibody [EPR23732-129] (ab259346)

        Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell) starved for 24 hours then treated with 100 ng/ml TPA for 4 hours (Red) /Untreated control (Green) cells labelling FRA1 with ab259346 at 1/500 dilution (0.1ug)/ red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab259346? Please let us know so that we can cite the reference in this datasheet.

      ab259346 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com