Recombinant Anti-NK-p44 antibody [EPR23732-129] (ab259346)
![Flow Cytometry - Anti-NK-p44 antibody [EPR23732-129] (ab259346)](https://www.abcam.com/ps/products/259/ab259346/Images/ab259346-1-anti-nkp44-antibody-epr23732129-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg "Flow Cytometry - Anti-NK-p44 antibody [EPR23732-129] (ab259346)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23732-129] to NK-p44
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-NK-p44 antibody [EPR23732-129]
See all NK-p44 primary antibodies -
Description
Rabbit monoclonal [EPR23732-129] to NK-p44 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC,IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human NK-p44 aa 1-200. The exact sequence is proprietary.
Database link: O95944 -
Positive control
- Flow Cyt: Human peripheral blood mononuclear cell (PBMC) treated with 20ng/ml Interleukin-2 (IL-2) for 7 days
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23732-129 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab259346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. |
Target
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Function
Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. -
Tissue specificity
Selectively expressed by activated NK cells and by in vitro cultured (i.e. activated) TCRg/d lymphoid cells. -
Sequence similarities
Belongs to the natural cytotoxicity receptor (NCR) family.
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9436 Human
- Omim: 604531 Human
- SwissProt: O95944 Human
- Unigene: 194721 Human
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Alternative names
- Activating NK receptor NKp44 antibody
- CD336 antibody
- CD336 antigen antibody
see all
Images
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Flow Cytometry - Anti-NK-p44 antibody [EPR23732-129] (ab259346)
Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell) starved for 24 hours then treated with 100 ng/ml TPA for 4 hours (Red) /Untreated control (Green) cells labelling FRA1 with ab259346 at 1/500 dilution (0.1ug)/ red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab259346 has not yet been referenced specifically in any publications.
