For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    nk-p44-antibody-epr50602-ab133668.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Adaptive Immunity T Cells Cytotoxic Cells
Share by email
RecombinantRabMAb

Recombinant Anti-NK-p44 antibody [EPR5060(2)] (ab133668)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
  • Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
  • OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
  • Anti-NK-p44 antibody [EPR5060(2)] (ab133668)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5060(2)] to NK-p44
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
Product image
Recombinant Human NK-p44 protein (denatured) (ab177598)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NK-p44 antibody [EPR5060(2)]
    See all NK-p44 primary antibodies
  • Description

    Rabbit monoclonal [EPR5060(2)] to NK-p44
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NK-p44 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Human lymph node lysate; K562, Jurkat, HuT-78 and Molt-4 cell lysates; K562 cells
  • General notes

    Previously labelled as NCR2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.00 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5060(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Signal Transduction
    • Adapters
    • Transmembrane

Associated products

  • Alternative Versions

    • Anti-NK-p44 antibody [EPR5060(2)] - BSA and Azide free (ab246800)
    • Anti-NK-p44 antibody [EPR5060(2)] - Low endotoxin, Azide free (ab246801)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat membrane extract lysate (ab30130)
    • MOLT4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human NK-p44 protein (denatured) (ab177598)

Applications

Our Abpromise guarantee covers the use of ab133668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 31 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
    • Tissue specificity

      Selectively expressed by activated NK cells and by in vitro cultured (i.e. activated) TCRg/d lymphoid cells.
    • Sequence similarities

      Belongs to the natural cytotoxicity receptor (NCR) family.
      Contains 1 Ig-like (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession O95944 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9436 Human
      • Omim: 604531 Human
      • SwissProt: O95944 Human
      • Unigene: 194721 Human
      • Alternative names

        • Activating NK receptor NKp44 antibody
        • CD336 antibody
        • CD336 antigen antibody
        • dJ149M18.1 antibody
        • LY95 antibody
        • Lymphocyte antigen 95 (activating NK receptor; NK p44) antibody
        • Lymphocyte antigen 95 homolog (activating NK receptor; NK p44) antibody
        • Lymphocyte antigen 95 homolog antibody
        • Lymphocyte antigen 95, mouse, homolog of antibody
        • Natural cytotoxicity triggering receptor 2 antibody
        • Natural killer cell p44 related protein antibody
        • Natural killer cell p44-related protein antibody
        • NCR2 antibody
        • NCTR2_HUMAN antibody
        • NK cell activating receptor (NKp44) antibody
        • NK cell activating receptor antibody
        • NK cell-activating receptor antibody
        • NK p44 antibody
        • NK-p44 antibody
        • NKp44 antibody
        see all

      Images

      • Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
        Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
        All lanes : Anti-NK-p44 antibody [EPR5060(2)] (ab133668) at 1/10000 dilution

        Lane 1 : Human lymph node lysate
        Lane 2 : K562 cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : HuT-78 cell lysate
        Lane 5 : Molt-4 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

        Predicted band size: 31 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
        Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)

        Immunofluorescent analysis of K562 cells labelling NK-p44 with ab133668 at 1/100 dilution.

      • OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
        OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
        Anti-NK-p44 antibody [EPR5060(2)] (ab133668)

      Protocols

      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (3)

      Publishing research using ab133668? Please let us know so that we can cite the reference in this datasheet.

      ab133668 has been referenced in 3 publications.

      • Adithan A  et al. Biological effects of cyclosporin A on CD3-CD161+ and CD3+CD161+ lymphocytes. Mol Cell Biochem N/A:N/A (2019). PubMed: 31020492
      • Qiu Y  et al. Lactobacillus plantarum Enhanced IL-22 Production in Natural Killer (NK) Cells That Protect the Integrity of Intestinal Epithelial Cell Barrier Damaged by Enterotoxigenic Escherichia coli. Int J Mol Sci 18:N/A (2017). PubMed: 29137183
      • Vourc'h M  et al. Exoenzyme T Plays a Pivotal Role in the IFN-? Production afterPseudomonasChallenge in IL-12 Primed Natural Killer Cells. Front Immunol 8:1283 (2017). PubMed: 29067027

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab133668.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.