Recombinant
RabMAb

Recombinant Anti-NK-p44 antibody [EPR5060(2)] (ab133668)

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Overview

  • Product name

    Anti-NK-p44 antibody [EPR5060(2)]
    See all NK-p44 primary antibodies

  • Description

    Rabbit monoclonal [EPR5060(2)] to NK-p44

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NK-p44 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Human lymph node lysate; K562, Jurkat, HuT-78 and Molt-4 cell lysates; K562 cells

  • General notes

    Previously labelled as NCR2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 31 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

    • Tissue specificity

      Selectively expressed by activated NK cells and by in vitro cultured (i.e. activated) TCRg/d lymphoid cells.

    • Sequence similarities

      Belongs to the natural cytotoxicity receptor (NCR) family.
      Contains 1 Ig-like (immunoglobulin-like) domain.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Activating NK receptor NKp44 antibody
        • CD336 antibody
        • CD336 antigen antibody
        • dJ149M18.1 antibody
        • LY95 antibody
        • Lymphocyte antigen 95 (activating NK receptor; NK p44) antibody
        • Lymphocyte antigen 95 homolog (activating NK receptor; NK p44) antibody
        • Lymphocyte antigen 95 homolog antibody
        • Lymphocyte antigen 95, mouse, homolog of antibody
        • Natural cytotoxicity triggering receptor 2 antibody
        • Natural killer cell p44 related protein antibody
        • Natural killer cell p44-related protein antibody
        • NCR2 antibody
        • NCTR2_HUMAN antibody
        • NK cell activating receptor (NKp44) antibody
        • NK cell activating receptor antibody
        • NK cell-activating receptor antibody
        • NK p44 antibody
        • NK-p44 antibody
        • NKp44 antibody
        see all

      Images

      • Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
        Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
        All lanes : Anti-NK-p44 antibody [EPR5060(2)] (ab133668) at 1/10000 dilution

        Lane 1 : Human lymph node lysate
        Lane 2 : K562 cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : HuT-78 cell lysate
        Lane 5 : Molt-4 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

        Predicted band size: 31 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
        Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)

        Immunofluorescent analysis of K562 cells labelling NK-p44 with ab133668 at 1/100 dilution.

      • Other - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
        Other - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:

      • Adithan A  et al. Biological effects of cyclosporin A on CD3-CD161+ and CD3+CD161+ lymphocytes. Mol Cell Biochem N/A:N/A (2019). Read more (PubMed: 31020492) »
      • Qiu Y  et al. Lactobacillus plantarum Enhanced IL-22 Production in Natural Killer (NK) Cells That Protect the Integrity of Intestinal Epithelial Cell Barrier Damaged by Enterotoxigenic Escherichia coli. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 29137183) »
      See all 3 Publications for this product

      Publishing research using ab133668? Please let us know so that we can cite the reference in this datasheet.

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