Recombinant Anti-NK-p44 antibody [EPR5060(2)] - Low endotoxin, Azide free (ab246801)
![Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] - Low endotoxin, Azide free (ab246801)](https://www.abcam.com/ps/products/246/ab246801/Images/ab246801-2-NCR2Primaryantibodiesab1336682.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] - Low endotoxin, Azide free (ab246801)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5060(2)] to NK-p44 - Low endotoxin, Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NK-p44 antibody [EPR5060(2)] - Low endotoxin, Azide free
See all NK-p44 primary antibodies -
Description
Rabbit monoclonal [EPR5060(2)] to NK-p44 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human NK-p44 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- Human lymph node lysate; K562, Jurkat, HuT-78 and Molt-4 cell lysates; K562 cells
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General notes
ab246801 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Previously labelled as NCR2.
Previously labelled as NCR2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.00 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Endotoxin level is less than 1 EU/ml as determined by the TAL test -
Clonality
Monoclonal -
Clone number
EPR5060(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab246801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. |
Target
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Function
Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. -
Tissue specificity
Selectively expressed by activated NK cells and by in vitro cultured (i.e. activated) TCRg/d lymphoid cells. -
Sequence similarities
Belongs to the natural cytotoxicity receptor (NCR) family.
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9436 Human
- Omim: 604531 Human
- SwissProt: O95944 Human
- Unigene: 194721 Human
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Alternative names
- Activating NK receptor NKp44 antibody
- CD336 antibody
- CD336 antigen antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] - Low endotoxin, Azide free (ab246801)
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![OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] - Low endotoxin, Azide free (ab246801)](https://www.abcam.com/ps/products/246/ab246801/Images/ab246801-1-NCR2Primaryantibodiesab1336683.jpg)
OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] - Low endotoxin, Azide free (ab246801)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133668).
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![Anti-NK-p44 antibody [EPR5060(2)] - Low endotoxin, Azide free (ab246801)](https://www.abcam.com/ps/products/246/ab246801/Images/ab246801-4-benefits-of-recombinant-antibodies.png)
Anti-NK-p44 antibody [EPR5060(2)] - Low endotoxin, Azide free (ab246801)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab246801 has not yet been referenced specifically in any publications.
