Anti-NKAP antibody (ab229096)
Key features and details
- Rabbit polyclonal to NKAP
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NKAP antibody -
Description
Rabbit polyclonal to NKAP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment within Human NKAP (internal sequence). The exact sequence is proprietary.
Database link: Q8N5F7 -
Positive control
- WB: Hela whole cell and nuclear extracts. IHC-P: Mouse duodenum tissue. Rat hind brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
Target
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Function
Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter. -
Sequence similarities
Belongs to the UPF0396 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79576 Human
- Entrez Gene: 67050 Mouse
- Entrez Gene: 298342 Rat
- Entrez Gene: 694891 Rhesus monkey
- Omim: 300766 Human
- SwissProt: Q8N5F7 Human
- SwissProt: Q9D0F4 Mouse
- SwissProt: Q4V7C9 Rat
see all -
Alternative names
- NF-kappa-B-activating protein antibody
- NFKB activating protein antibody
- Nkap antibody
see all
Images
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Paraffin-embedded rat hind brain tissue stained for NKAP using ab229096 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-NKAP antibody (ab229096) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 47 kDa10% SDS-PAGE gel.
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Paraffin-embedded mouse duodenum tissue stained for NKAP using ab229096 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab229096 has been referenced in 7 publications.
- von Elsner L et al. Biallelic FRA10AC1 variants cause a neurodevelopmental disorder with growth retardation. Brain 145:1551-1563 (2022). PubMed: 34694367
- Sun S et al. RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner. Cell Death Dis 13:73 (2022). PubMed: 35064112
- Liu J et al. Nuclear Factor-?B Activating Protein Plays an Oncogenic Role in Neuroblastoma Tumorigenesis and Recurrence Through the Phosphatidylinositol 3-Kinase/Protein Kinase B Signaling Pathway. Front Cell Dev Biol 8:622793 (2020). PubMed: 33553160
- Ma Q et al. NKAP promotes renal cell carcinoma growth via AKT/mTOR signalling pathway. Cell Biochem Funct 38:574-581 (2020). PubMed: 32032976
- Li F et al. NKAP functions as an oncogene in Ewing sarcoma cells partly through the AKT signaling pathway. Exp Ther Med 18:3037-3045 (2019). PubMed: 31555387
- Song T et al. NKAP plays an oncogenic function partly through AKT signaling pathway in hepatocellular carcinoma. Neoplasma 66:792-800 (2019). PubMed: 31305121
- Shu W et al. The oncogenic role of NKAP in the growth and invasion of colon cancer cells. Oncol Rep 42:2130-2138 (2019). PubMed: 31545474