Key features and details
- Rabbit polyclonal to NKAP
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NKAP antibody
See all NKAP primary antibodies
DescriptionRabbit polyclonal to NKAP
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human NKAP (internal sequence). The exact sequence is proprietary.
Database link: Q8N5F7
- WB: Hela whole cell and nuclear extracts. IHC-P: Mouse duodenum tissue. Rat hind brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 47 kDa.|
FunctionActs as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter.
Sequence similaritiesBelongs to the UPF0396 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- NF-kappa-B-activating protein antibody
- NFKB activating protein antibody
- Nkap antibody
Paraffin-embedded rat hind brain tissue stained for NKAP using ab229096 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-NKAP antibody (ab229096) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 47 kDa
10% SDS-PAGE gel.
Paraffin-embedded mouse duodenum tissue stained for NKAP using ab229096 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229096 has been referenced in 1 publication.
- Li F et al. NKAP functions as an oncogene in Ewing sarcoma cells partly through the AKT signaling pathway. Exp Ther Med 18:3037-3045 (2019). PubMed: 31555387