Anti-NKCC1 antibody (ab99558)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-NKCC1 antibody
See all NKCC1 primary antibodies -
Description
Goat polyclonal to NKCC1 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Cynomolgus monkey
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Immunogen
Synthetic peptide:
C-NALQHSIRLSGVEDH
, corresponding to internal sequence amino acids 769-783 of Human NKCC1 (NP_001037.1.) -
Positive control
- WB: Human, rat and mouse skeletal muscle, heart and brain tissue lysates. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Ammonium sulphate precipitation followed by antigen affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab99558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ELISA | 1/64000. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Function
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. -
Tissue specificity
Expressed in many tissues. -
Sequence similarities
Belongs to the SLC12A transporter family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 286845 Cow
- Entrez Gene: 481490 Dog
- Entrez Gene: 6558 Human
- Entrez Gene: 20496 Mouse
- Entrez Gene: 83629 Rat
- Omim: 600840 Human
- SwissProt: P55011 Human
- SwissProt: P55012 Mouse
see all -
Alternative names
- Basolateral Na-K-Cl symporter antibody
- BSC antibody
- BSC2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NKCC1 antibody (ab99558)ICC/IF image of ab99558 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab99558, 5ug/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody (ab99558)ab99558 (3.8µg/ml) staining of paraffin embedded Human Colon shows membranous staining. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Datasheets and documents
References
This product has been referenced in:
- Ma H et al. NKCC1 promotes EMT-like process in GBM via RhoA and Rac1 signaling pathways. J Cell Physiol 234:1630-1642 (2019). Read more (PubMed: 30159893) »
