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  1. Link

    nkcc1-antibody-ab99558.pdf

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Cardiovascular Hypoxia Associated Proteins
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Anti-NKCC1 antibody (ab99558)

  • Datasheet
Reviews (1)Q&A (1)References (4)

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Promotion Information

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Key features and details

  • Goat polyclonal to NKCC1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Protein
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Recombinant Human NKCC1 protein (ab159532)
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Anti-KCC2 antibody (ab97502)

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Overview

  • Product name

    Anti-NKCC1 antibody
    See all NKCC1 primary antibodies
  • Description

    Goat polyclonal to NKCC1
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human NKCC1 aa 750-850 (internal sequence). NP_001037.1
    Database link: P55011

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Ammonium sulphate precipitation followed by antigen affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Hypoxia
    • Associated Proteins
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Neuroscience
    • Neurotransmitter
    • Transporters
    • Other Transporters
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • Rat brain normal tissue lysate - membrane extract (ab29473)
  • Recombinant Protein

    • Recombinant Human NKCC1 protein (ab159532)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab99558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
  • Tissue specificity

    Expressed in many tissues.
  • Sequence similarities

    Belongs to the SLC12A transporter family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P55011 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 286845 Cow
    • Entrez Gene: 481490 Dog
    • Entrez Gene: 6558 Human
    • Entrez Gene: 20496 Mouse
    • Entrez Gene: 83629 Rat
    • Omim: 600840 Human
    • SwissProt: P55011 Human
    • SwissProt: P55012 Mouse
    • Unigene: 162585 Human
    • Unigene: 712970 Human
    • Unigene: 399997 Mouse
    see all
  • Alternative names

    • Basolateral Na-K-Cl symporter antibody
    • BSC antibody
    • BSC2 antibody
    • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 antibody
    • mBSC2 antibody
    • MGC104233 antibody
    • NKCC1 antibody
    • PPP1R141 antibody
    • Protein phosphatase 1, regulatory subunit 141 antibody
    • S12A2_HUMAN antibody
    • Slc12a2 antibody
    • sodium-potassium-chloride cotransporter 1 antibody
    • Solute carrier family 12 (sodium/potassium/chloride transporter), member 2 antibody
    • solute carrier family 12 (sodium/potassium/chloride transporters) member 2 antibody
    • Solute carrier family 12 member 2 antibody
    • sy-ns antibody
    see all

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (4)

Publishing research using ab99558? Please let us know so that we can cite the reference in this datasheet.

ab99558 has been referenced in 4 publications.

  • He D  et al. Asthmatic Airway Vagal Hypertonia Involves Chloride Dyshomeostasis of Preganglionic Neurons in Rats. Front Neurosci 14:31 (2020). PubMed: 32082109
  • McNeill JK  et al. The Excitatory Effects of GABA within the Suprachiasmatic Nucleus: Regulation of Na-K-2Cl Cotransporters (NKCCs) by Environmental Lighting Conditions. J Biol Rhythms 35:275-286 (2020). PubMed: 32406304
  • Ma H  et al. NKCC1 promotes EMT-like process in GBM via RhoA and Rac1 signaling pathways. J Cell Physiol 234:1630-1642 (2019). PubMed: 30159893
  • Rohr KE  et al. Seasonal plasticity in GABAA signaling is necessary for restoring phase synchrony in the master circadian clock network. Elife 8:N/A (2019). PubMed: 31746738

Customer reviews and Q&As

Show All Reviews Q&A
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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NKCC1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Salivary gland)
Specification
Salivary gland
Permeabilization
No
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

Submitted Nov 03 2014

Question

No signal in ELISA with mouse brain tissue lysate.

Read More

Abcam community

Verified customer

Asked on Dec 16 2011

Answer

Thank you for your call today and for letting us know about the trouble with this antibody. As we discussed, I am sending a free of charge replacement vial on the order ***, which should arrive around January 3. Please keep me updated about the results with this antibody, and let me know if there is anything else that we can do for you.

Read More

Abcam Scientific Support

Answered on Dec 16 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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