- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-NKCC1 antibody
See all NKCC1 primary antibodies -
DescriptionGoat polyclonal to NKCC1
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Host speciesGoat
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Tested applicationsSuitable for: IHC-P, ELISA, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Cynomolgus monkey
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Immunogen
Synthetic peptide:
C-NALQHSIRLSGVEDH
, corresponding to internal sequence amino acids 769-783 of Human NKCC1 (NP_001037.1.) -
Positive control
- WB: Human, rat and mouse skeletal muscle, heart and brain tissue lysates. ICC/IF: HepG2 cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesAmmonium sulphate precipitation followed by antigen affinity chromatography.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab99558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ELISA | 1/64000. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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FunctionElectrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
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Tissue specificityExpressed in many tissues.
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Sequence similaritiesBelongs to the SLC12A transporter family.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 286845 Cow
- Entrez Gene: 481490 Dog
- Entrez Gene: 6558 Human
- Entrez Gene: 20496 Mouse
- Entrez Gene: 83629 Rat
- Omim: 600840 Human
- SwissProt: P55011 Human
- SwissProt: P55012 Mouse
see all -
Alternative names
- Basolateral Na-K-Cl symporter antibody
- BSC antibody
- BSC2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NKCC1 antibody (ab99558)ICC/IF image of ab99558 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab99558, 5ug/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody (ab99558)ab99558 (3.8µg/ml) staining of paraffin embedded Human Colon shows membranous staining. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
References
ab99558 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"