Anti-NKCC1 antibody (ab99558)
Key features and details
- Goat polyclonal to NKCC1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NKCC1 antibody
See all NKCC1 primary antibodies -
Description
Goat polyclonal to NKCC1 -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Cynomolgus monkey
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Immunogen
Synthetic peptide:
C-NALQHSIRLSGVEDH
, corresponding to internal sequence amino acids 769-783 of Human NKCC1 (NP_001037.1.) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Ammonium sulphate precipitation followed by antigen affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab99558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. -
Tissue specificity
Expressed in many tissues. -
Sequence similarities
Belongs to the SLC12A transporter family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 286845 Cow
- Entrez Gene: 481490 Dog
- Entrez Gene: 6558 Human
- Entrez Gene: 20496 Mouse
- Entrez Gene: 83629 Rat
- Omim: 600840 Human
- SwissProt: P55011 Human
- SwissProt: P55012 Mouse
see all -
Alternative names
- Basolateral Na-K-Cl symporter antibody
- BSC antibody
- BSC2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody (ab99558)ab99558 (3.8µg/ml) staining of paraffin embedded Human Colon shows membranous staining. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Datasheets and documents
References (2)
ab99558 has been referenced in 2 publications.
- Ma H et al. NKCC1 promotes EMT-like process in GBM via RhoA and Rac1 signaling pathways. J Cell Physiol 234:1630-1642 (2019). PubMed: 30159893
- Rohr KE et al. Seasonal plasticity in GABAA signaling is necessary for restoring phase synchrony in the master circadian clock network. Elife 8:N/A (2019). PubMed: 31746738
