Overview

  • Product name

    Anti-NKD2 antibody
  • Description

    Mouse polyclonal to NKD2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    NKD2 full length human protein

  • Positive control

    • NKD2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.
  • Tissue specificity

    Expressed in kidney, lung, pancreas and spleen.
  • Sequence similarities

    Belongs to the NKD family.
    Contains 1 EF-hand domain.
  • Developmental stage

    Expressed in fetal kidney and lung.
  • Domain

    The N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.
  • Post-translational
    modifications

    Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.
  • Cellular localization

    Cell membrane. Cytoplasm. Cytoplasmic vesicle.
  • Information by UniProt
  • Database links

  • Alternative names

    • hNkd2 antibody
    • Naked 2 antibody
    • Naked-2 antibody
    • nkd2 antibody
    • NKD2_HUMAN antibody
    • Protein naked cuticle homolog 2 antibody
    see all

Images

  • All lanes : Anti-NKD2 antibody (ab67911) at 1/500 dilution

    Lane 1 : NKD2 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size: 50 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

References

ab67911 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting Abcam. The antibody ab67911 has not been tested in IHC and so I cannot provide any protocol information for you. As it has not been tested in IHC, I can offer you our testing discount. The details of which are below. If there is anything else I can help you with, please let me know. Testing Discount Details To our knowledge, ab67911has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab6791 now, test it in IHC and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab67911 in IHC. I will then send a discount code. This code must be issued before purchasing ab67911so please wait for my reply before ordering. 2. Purchase ab67911 either by phone, fax, or online (www.abcam.com). 3. Test it in IHC. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any  primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab67911 turns out to be unsuitable for IHC, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.    

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