Key features and details
- Mouse polyclonal to NKD2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NKD2 antibody
DescriptionMouse polyclonal to NKD2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
NKD2 full length human protein
- NKD2 transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 50 kDa).|
FunctionCell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.
Tissue specificityExpressed in kidney, lung, pancreas and spleen.
Sequence similaritiesBelongs to the NKD family.
Contains 1 EF-hand domain.
Developmental stageExpressed in fetal kidney and lung.
DomainThe N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.
modificationsUbiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.
Cellular localizationCell membrane. Cytoplasm. Cytoplasmic vesicle.
- Information by UniProt
- hNkd2 antibody
- Naked 2 antibody
- Naked-2 antibody
All lanes : Anti-NKD2 antibody (ab67911) at 1/500 dilution
Lane 1 : NKD2 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 50 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
ab67911 has not yet been referenced specifically in any publications.