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Synthetic peptide within Human NKD2 aa 373-401 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q969F2
Our Abpromise guarantee covers the use of ab170804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Flow cytometric analysis of Jurkat cells labeling NKD2 using ab170804 at a 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used.
Immunocytochemistry/ Immunofluorescence analysis of A435 cells labeling NKD2 with ab170804 at a 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human kidney tissue labeling NKD2 with ab170804 at a 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"