Recombinant
RabMAb

Recombinant Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free (ab241456)

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Overview

  • Product name

    Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR22137] to NKG2A + NKG2E + NKG2C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human NKG2A aa 100 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human natural killer cell lymphoma tissue.

  • General notes

    Ab241456 is the carrier-free version of ab224699. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab241456 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab241456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 34-38 kDa (predicted molecular weight: 26 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free (ab241456)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free (ab241456)

    Immunohistochemical analysis of paraffin-embedded human natural killer cell lymphoma tissue labeling NKG2A + NKG2E + NKG2C with ab224699 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human natural killer-cell lymphoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224699).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab241456 has not yet been referenced specifically in any publications.

Publishing research using ab241456? Please let us know so that we can cite the reference in this datasheet.

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