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RecombinantRabMAb

Recombinant Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free (ab241456)

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  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free (ab241456)
  • Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free (ab241456)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22137] to NKG2A + NKG2E + NKG2C - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Recombinant Human NKG2A protein (His tag) (ab215651)
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Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)

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Overview

  • Product name

    Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free
  • Description

    Rabbit monoclonal [EPR22137] to NKG2A + NKG2E + NKG2C - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NKG2A aa 100 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human natural killer cell lymphoma tissue.
  • General notes

    Ab241456 is the carrier-free version of ab224699. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab241456 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22137
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Immunology
    • Cell Type Markers
    • CD
    • NK Cells
    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I
    • Immunology
    • Innate Immunity
    • NK Cells

Associated products

  • Alternative Versions

    • Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] (ab224699)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunohistochemistry reagents

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human NKG2A protein (His tag) (ab215651)

Applications

Our Abpromise guarantee covers the use of ab241456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 34-38 kDa (predicted molecular weight: 26 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Cellular localization

    NKG2A: Membrane. NKG2E: Membrane. NKG2C: Membrane.
  • Database links

    • Entrez Gene: 3821 Human
    • Entrez Gene: 3822 Human
    • Entrez Gene: 3823 Human
    • Omim: 161555 Human
    • Omim: 602891 Human
    • Omim: 602892 Human
    • SwissProt: P26715 Human
    • SwissProt: P26717 Human
    • SwissProt: Q07444 Human
    • Unigene: 512576 Human
    • Unigene: 591157 Human
    • Unigene: 654362 Human
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free (ab241456)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free (ab241456)

    Immunohistochemical analysis of paraffin-embedded human natural killer cell lymphoma tissue labeling NKG2A + NKG2E + NKG2C with ab224699 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human natural killer-cell lymphoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224699).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free (ab241456)
    Anti-NKG2A + NKG2E + NKG2C antibody [EPR22137] - BSA and Azide free (ab241456)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab241456? Please let us know so that we can cite the reference in this datasheet.

    ab241456 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab241456.
    Please use the links above to contact us or submit feedback about this product.

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