Application
Immunohistochemistry (Frozen sections)
Sample
African green monkey Tissue sections (Ileum)
Permeabilization
Yes - Triton X-100, 0.2%, 45min
Specification
Ileum
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.05% Tween-20
Dilution
1/250
Additional data
Additional Notes
Tested Antibody in Red.
Co-stain with Santa Cruz goat polyclonal anti-NKG2A (sc-9616) in Green. This antibody has previously been validated by our lab.
Lack of colocalization seems to show that the abcam mouse-anti-NKG2A-Antibody is not suitable for IHC in AGM.
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Submitted Dec 17 2018