Anti-NKG2A antibody (ab96319)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NKG2A antibody
See all NKG2A primary antibodies -
Description
Rabbit polyclonal to NKG2A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NKG2A (N terminal). The exact sequence is proprietary. Carrier protein conjugated.
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Positive control
- WB: A431 and HepG2 cell lysate. ICC/IF: HeLa cells. IHC-P: H1299 xenograft.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab96319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
Target
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Function
Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. -
Tissue specificity
Natural killer cells. -
Sequence similarities
Contains 1 C-type lectin domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3821 Human
- Omim: 161555 Human
- SwissProt: P26715 Human
- Unigene: 512576 Human
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Alternative names
- C lectin type II protein antibody
- CD159 antigen like family member A antibody
- CD159 antigen-like family member A antibody
see all
Images
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Western blot - Anti-NKG2A antibody (ab96319)All lanes : Anti-NKG2A antibody (ab96319) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-NKG2A antibody (ab96319)Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeled for NKG2A using ab96319 at a 1/200 dilution in immunocytochemistry.
Bottom panel co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A antibody (ab96319)Paraffin-embedded H1299 xenograft stained for NKG2A using ab96319 at a 1/500 dilution in immunohistochemistry.
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15 mins.
Protocols
References
ab96319 has not yet been referenced specifically in any publications.
