Anti-NKG2A antibody (ab96319)
Key features and details
- Rabbit polyclonal to NKG2A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NKG2A antibody
See all NKG2A primary antibodies -
Description
Rabbit polyclonal to NKG2A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NKG2A (N terminal). The exact sequence is proprietary. Carrier protein conjugated.
-
Positive control
- WB: A431 and HepG2 cell lysate. ICC/IF: HeLa cells. IHC-P: H1299 xenograft.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab96319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
Target
-
Function
Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. -
Tissue specificity
Natural killer cells. -
Sequence similarities
Contains 1 C-type lectin domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3821 Human
- Omim: 161555 Human
- SwissProt: P26715 Human
- Unigene: 512576 Human
-
Alternative names
- C lectin type II protein antibody
- CD159 antigen like family member A antibody
- CD159 antigen-like family member A antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A antibody (ab96319)
Paraffin-embedded H1299 xenograft stained for NKG2A using ab96319 at a 1/500 dilution in immunohistochemistry.
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15 mins.
-
Western blot - Anti-NKG2A antibody (ab96319)All lanes : Anti-NKG2A antibody (ab96319) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-NKG2A antibody (ab96319)Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeled for NKG2A using ab96319 at a 1/200 dilution in immunocytochemistry.
Bottom panel co-stained with Hoechst 33342.
References (0)
ab96319 has not yet been referenced specifically in any publications.
