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Immunology Cell Type Markers CD NK Cells
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Anti-NKG2A antibody (ab96319)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A antibody (ab96319)
  • Western blot - Anti-NKG2A antibody (ab96319)
  • Immunocytochemistry/ Immunofluorescence - Anti-NKG2A antibody (ab96319)

Key features and details

  • Rabbit polyclonal to NKG2A
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human NKG2A protein (His tag) (ab215651)

View more associated products

Overview

  • Product name

    Anti-NKG2A antibody
    See all NKG2A primary antibodies
  • Description

    Rabbit polyclonal to NKG2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NKG2A (N terminal). The exact sequence is proprietary. Carrier protein conjugated.

  • Positive control

    • WB: A431 and HepG2 cell lysate. ICC/IF: HeLa cells. IHC-P: H1299 xenograft.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • NK Cells

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human NKG2A protein (His tag) (ab215651)

Applications

Our Abpromise guarantee covers the use of ab96319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.

Target

  • Function

    Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.
  • Tissue specificity

    Natural killer cells.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P26715 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3821 Human
    • Omim: 161555 Human
    • SwissProt: P26715 Human
    • Unigene: 512576 Human
    • Alternative names

      • C lectin type II protein antibody
      • CD159 antigen like family member A antibody
      • CD159 antigen-like family member A antibody
      • CD159a antibody
      • CD159a antigen antibody
      • Killer cell lectin like receptor subfamily C member 1 antibody
      • KLRC1 antibody
      • MGC13374 antibody
      • MGC59791 antibody
      • Natural killer cell lectin antibody
      • Natural killer cell protein group 2-A1 antibody
      • Natural killer cell receptor NKG2A antibody
      • Natural killer group protein 2 antibody
      • NK cell receptor A antibody
      • NKG2 1/B activating NK receptor antibody
      • NKG2 A antibody
      • NKG2 A/B activating NK receptor antibody
      • NKG2 A/B type II integral membrane protein antibody
      • NKG2 A/NKG2 B type II integral membrane protein antibody
      • NKG2 antibody
      • NKG2 B antibody
      • NKG2-1/B activating NK receptor antibody
      • NKG2-A/B-activating NK receptor antibody
      • NKG2-A/NKG2-B type II integral membrane protein antibody
      • NKG2A antibody
      • NKG2A_HUMAN antibody
      • NKG2B antibody
      • rNKG2A antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A antibody (ab96319)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A antibody (ab96319)

      Paraffin-embedded H1299 xenograft stained for NKG2A using ab96319 at a 1/500 dilution in immunohistochemistry.

      Antigen Retrieval: EDTA based, pH 8.0 buffer, 15 mins.

    • Western blot - Anti-NKG2A antibody (ab96319)
      Western blot - Anti-NKG2A antibody (ab96319)
      All lanes : Anti-NKG2A antibody (ab96319) at 1/1000 dilution

      Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
      Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 26 kDa



      12% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-NKG2A antibody (ab96319)
      Immunocytochemistry/ Immunofluorescence - Anti-NKG2A antibody (ab96319)

      Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeled for NKG2A using ab96319 at a 1/200 dilution in immunocytochemistry.

      Bottom panel co-stained with Hoechst 33342.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab96319? Please let us know so that we can cite the reference in this datasheet.

    ab96319 has not yet been referenced specifically in any publications.

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