Overview

  • Product name

    Anti-NKG2C antibody - N-terminal
    See all NKG2C primary antibodies
  • Description

    Rabbit polyclonal to NKG2C - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NKG2C aa 1-30 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P26717

  • Positive control

    • WB: CEM, K562 and HL-60 cell lysates. IHC-P: Human colon tissue. Flow Cytometry: HL-60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 26 kDa.
Flow Cyt 1/10 - 1/50.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.
  • Tissue specificity

    Natural killer cells.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD antigen CD159c antibody
    • CD159 antigen like family member C antibody
    • CD159 antigen-like family member C antibody
    • CD159c antibody
    • Killer cell lectin like receptor subfamily C, member 2 antibody
    • KLRC2 antibody
    • NK cell receptor C antibody
    • NKG2-C type II integral membrane protein antibody
    • NKG2-C-activating NK receptor antibody
    • NKG2C activating NK receptor antibody
    • NKG2C antibody
    • NKG2C_HUMAN antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human colon tissue stained for NKG2C with ab230900 at 1/50 dilution in immunohistochemical analysis.

  • Anti-NKG2C antibody - N-terminal (ab230900) at 1/1000 dilution + CEM cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 26 kDa

  • Anti-NKG2C antibody - N-terminal (ab230900) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 26 kDa

  • Anti-NKG2C antibody - N-terminal (ab230900) at 1/1000 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 26 kDa

  • Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling NKG2C with ab230900 at 1/10 dilution (green) compared with a negative control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used.

References

ab230900 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230900.
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