Overview

  • Product name

  • Description

    Goat polyclonal to NKG2D
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    KVYSKEDQDLLK

    , corresponding to Internal sequence amino acids 136-147 of Human NKG2D

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 0.05% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 25.3 kDa).

Target

  • Function

    Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.
  • Tissue specificity

    Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs).
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD314 antibody
    • CD314 antigen antibody
    • D12S2489E antibody
    • Killer cell lectin like receptor subfamily K member 1 antibody
    • Killer cell lectin-like receptor subfamily K member 1 antibody
    • KLR antibody
    • KLRC4 KLRK1 readthrough antibody
    • KLRK1 antibody
    • NK cell receptor D antibody
    • NK lectin-like receptor antibody
    • NKG2 D activating NK receptor antibody
    • NKG2 D type II integral membrane protein antibody
    • NKG2-D antibody
    • NKG2-D type II integral membrane protein antibody
    • NKG2-D-activating NK receptor antibody
    • Nkg2d antibody
    • NKG2D_HUMAN antibody
    • NKLLR antibody
    • NKR P2 antibody
    • Nkrp2 antibody
    see all

Images

  • Anti-NKG2D antibody (ab36136) at 0.3 µg/ml + Human Spleen lysate (RIPA buffer, 30µg total protein per lane).

    Predicted band size: 25.3 kDa



    ab36136 staining (0.3µg/ml) of Human Spleen lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References

This product has been referenced in:

  • Kamei R  et al. Expression levels of UL16 binding protein 1 and natural killer group 2 member D affect overall survival in patients with gastric cancer following gastrectomy. Oncol Lett 15:747-754 (2018). Read more (PubMed: 29391893) »
  • Park YP  et al. Complex regulation of human NKG2D-DAP10 cell surface expression: opposing roles of the {gamma}c cytokines and TGF-{beta}1. Blood 118:3019-27 (2011). WB ; Human . Read more (PubMed: 21816829) »
See all 2 Publications for this product

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