Recombinant

Recombinant Anti-NKG2D antibody [EPR21224] (ab233698)

Overview

  • Product name

    Anti-NKG2D antibody [EPR21224]
    See all NKG2D primary antibodies
  • Description

    Rabbit monoclonal [EPR21224] to NKG2D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • Flow Cyt: Human PBMCs.
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21224
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 10 µg/ml.

Target

  • Function

    Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.
  • Tissue specificity

    Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs).
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD314 antibody
    • CD314 antigen antibody
    • D12S2489E antibody
    • Killer cell lectin like receptor subfamily K member 1 antibody
    • Killer cell lectin-like receptor subfamily K member 1 antibody
    • KLR antibody
    • KLRC4 KLRK1 readthrough antibody
    • KLRK1 antibody
    • NK cell receptor D antibody
    • NK lectin-like receptor antibody
    • NKG2 D activating NK receptor antibody
    • NKG2 D type II integral membrane protein antibody
    • NKG2-D antibody
    • NKG2-D type II integral membrane protein antibody
    • NKG2-D-activating NK receptor antibody
    • Nkg2d antibody
    • NKG2D_HUMAN antibody
    • NKLLR antibody
    • NKR P2 antibody
    • Nkrp2 antibody
    see all

Images

  • Human PBMCs were incubated with anti-NKG2D (CD314) antibody ab233698 at 10µg/ml for 30 mins at 4°C. The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 dilution for 30 mins at 4°C. The cells were simultaneously stained with CD56 PE-conjugated (ab218637).

    Acquisition of >30,000 total events were collected. Gating strategy - live peripheral blood lymphocytes.

References

ab233698 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233698.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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