Overview

  • Product name

    Anti-NKIRAS1 antibody [ABM17B5]
    See all NKIRAS1 primary antibodies
  • Description

    Mouse monoclonal [ABM17B5] to NKIRAS1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human NKIRAS1 aa 90-180.
    Sequence:

    NLESFQRVELLKKEIDKFKDKKEVAIVVLGNKIDLSEQRQVDAEVAQQWA KSEKVRLWEVTVTDRKTLIEPFTLLASKLSQPQSKSSFPLP


    Database link: Q9NYS0

  • Positive control

    • WB: Jurkat, HepG2 and HeLa cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    ABM17B5
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 6 - 8 µg/ml. Predicted molecular weight: 22 kDa.

Target

  • Function

    Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.
  • Domain

    In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Leu residues, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2400004O09Rik antibody
    • I kappa B interacting Ras like protein 1 antibody
    • I-kappa-B-interacting Ras-like protein 1 antibody
    • Kappa B Ras 1 antibody
    • Kappa B Ras protein 1 antibody
    • Kappa B-Ras protein 1 antibody
    • KappaB Ras1 antibody
    • KappaB-Ras1 antibody
    • KBRAS 1 antibody
    • KBRAS1 antibody
    • KBRS1_HUMAN antibody
    • NF kappa B inhibitor interacting Ras like protein 1 antibody
    • NF-kappa-B inhibitor-interacting Ras-like protein 1 antibody
    • NFKB inhibitor interacting Ras like 1 antibody
    • NFKB inhibitor interacting Ras like protein 1 antibody
    • NKIRAS 1 antibody
    • nkiras1 antibody
    see all

Images

  • All lanes : Anti-NKIRAS1 antibody [ABM17B5] (ab228443) at 6 µg/ml

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Predicted band size: 22 kDa

References

ab228443 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228443.
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