Overview

  • Product name

  • Description

    Rabbit polyclonal to NKIRAS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-191 of Human NKIRAS2 (Uniprot ID: Q9NYR9).

  • Positive control

    • A549, HeLa and HCT116 whole cell lysate; Human MCF7 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.
  • Domain

    In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Ala and Leu residues, respectively, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZP434N1526 antibody
    • I kappa B interacting Ras like protein 2 antibody
    • I-kappa-B-interacting Ras-like protein 2 antibody
    • Kappa B Ras protein 2 antibody
    • Kappa B-Ras protein 2 antibody
    • KappaB Ras2 antibody
    • KappaB-Ras2 antibody
    • KBRAS 2 antibody
    • KBRAS2 antibody
    • KBRS2_HUMAN antibody
    • MGC74742 antibody
    • NF kappa B inhibitor interacting Ras like protein 2 antibody
    • NF-kappa-B inhibitor-interacting Ras-like protein 2 antibody
    • NFKB inhibitor interacting Ras like 2 antibody
    • NFKB inhibitor interacting Ras like protein 2 antibody
    • NKIRAS 2 antibody
    • NKIRAS2 antibody
    see all

Images

  • Anti-NKIRAS2 antibody (ab154020) at 1/500 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 22 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human MCF7 xenograft tissue labeling NKIRAS2 with ab154020 at 1/100 dilution.

References

This product has been referenced in:

  • Xu YJ  et al. Epstein-Barr virus-coded miR-BART13 promotes nasopharyngeal carcinoma cell growth and metastasis via targeting of the NKIRAS2/NF-?B pathway. Cancer Lett 447:33-40 (2019). Read more (PubMed: 30684592) »
See 1 Publication for this product

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