Anti-NKIRAS2 antibody - C-terminal (ab153988)

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Western blot - Anti-NKIRAS2 antibody - C-terminal (ab153988)
  • Immunocytochemistry/ Immunofluorescence - Anti-NKIRAS2 antibody - C-terminal (ab153988)

Key features and details

  • Rabbit polyclonal to NKIRAS2 - C-terminal
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-NKIRAS2 antibody - C-terminal
    See all NKIRAS2 primary antibodies

  • Description

    Rabbit polyclonal to NKIRAS2 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Monkey

  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 129-191 of Human NKIRAS2 (Uniprot ID Q9NYR9).

  • Positive control

    • 293T; A431; H1299; HeLa; HepG2; MOLT4 and Raji cell lysates; A431 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 22 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 22 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.

  • Tissue specificity

    Widely expressed.

  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.

  • Domain

    In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Ala and Leu residues, respectively, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DKFZP434N1526 antibody
    • I kappa B interacting Ras like protein 2 antibody
    • I-kappa-B-interacting Ras-like protein 2 antibody
    • Kappa B Ras protein 2 antibody
    • Kappa B-Ras protein 2 antibody
    • KappaB Ras2 antibody
    • KappaB-Ras2 antibody
    • KBRAS 2 antibody
    • KBRAS2 antibody
    • KBRS2_HUMAN antibody
    • MGC74742 antibody
    • NF kappa B inhibitor interacting Ras like protein 2 antibody
    • NF-kappa-B inhibitor-interacting Ras-like protein 2 antibody
    • NFKB inhibitor interacting Ras like 2 antibody
    • NFKB inhibitor interacting Ras like protein 2 antibody
    • NKIRAS 2 antibody
    • NKIRAS2 antibody
    see all

Images

  • Western blot - Anti-NKIRAS2 antibody - C-terminal (ab153988)
    Western blot - Anti-NKIRAS2 antibody - C-terminal (ab153988)
    All lanes : Anti-NKIRAS2 antibody - C-terminal (ab153988) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 22 kDa



    12% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-NKIRAS2 antibody - C-terminal (ab153988)
    Immunocytochemistry/ Immunofluorescence - Anti-NKIRAS2 antibody - C-terminal (ab153988)
    Immunofluorescence analysis of methanol-fixed A431 cells labeling NKIRAS2 using ab153988 at 1/200 dilution. Right panel costained with Hoechst 33342.

References (0)

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ab153988 has not yet been referenced specifically in any publications.

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