Anti-NKIRAS2 antibody - C-terminal (ab153988)
Overview
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Product name
Anti-NKIRAS2 antibody - C-terminal
See all NKIRAS2 primary antibodies -
Description
Rabbit polyclonal to NKIRAS2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Monkey
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Immunogen
Synthetic peptide, corresponding to a region within C terminal amino acids 129-191 of Human NKIRAS2 (Uniprot ID Q9NYR9).
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Positive control
- 293T; A431; H1299; HeLa; HepG2; MOLT4 and Raji cell lysates; A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- Recombinant Human NKIRAS2 protein (ab124599)
- HeLa whole cell lysate (ab150035)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- A431 whole cell lysate (ab30132)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
- MOLT4 whole cell lysate (ab7912)
- 293T whole cell lysate (ab95494)
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab153988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 22 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily. -
Domain
In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Ala and Leu residues, respectively, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 509053 Cow
- Entrez Gene: 28511 Human
- Entrez Gene: 71966 Mouse
- Entrez Gene: 287707 Rat
- Omim: 604497 Human
- SwissProt: Q9NYR9 Human
- SwissProt: Q9CR56 Mouse
- Unigene: 632252 Human
see all -
Alternative names
- DKFZP434N1526 antibody
- I kappa B interacting Ras like protein 2 antibody
- I-kappa-B-interacting Ras-like protein 2 antibody
see all
Images
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Western blot - Anti-NKIRAS2 antibody - C-terminal (ab153988)All lanes : Anti-NKIRAS2 antibody - C-terminal (ab153988) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-NKIRAS2 antibody - C-terminal (ab153988)Immunofluorescence analysis of methanol-fixed A431 cells labeling NKIRAS2 using ab153988 at 1/200 dilution. Right panel costained with Hoechst 33342.
Protocols
References
ab153988 has not yet been referenced specifically in any publications.
